Results 1 - 20 of 21 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30478 | 5' | -64.2 | NC_006548.1 | + | 18258 | 0.66 | 0.242959 |
Target: 5'- gUCCUCcgcguaGG-CGCUGaCUGAGCACUGGc -3' miRNA: 3'- -AGGAGc-----CCgGCGACgGGCUCGUGGCC- -5' |
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30478 | 5' | -64.2 | NC_006548.1 | + | 35208 | 0.66 | 0.242958 |
Target: 5'- aUCgUUGGGCCGCuggacgucaUGUUCGGcGCcCCGGa -3' miRNA: 3'- -AGgAGCCCGGCG---------ACGGGCU-CGuGGCC- -5' |
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30478 | 5' | -64.2 | NC_006548.1 | + | 27284 | 0.66 | 0.242959 |
Target: 5'- gCCUCGGcaGCgCGUUGCUCGGuCGCCGc -3' miRNA: 3'- aGGAGCC--CG-GCGACGGGCUcGUGGCc -5' |
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30478 | 5' | -64.2 | NC_006548.1 | + | 31904 | 0.66 | 0.231088 |
Target: 5'- aCCUCGaa-CGCUG-CUGAGCGCCGa -3' miRNA: 3'- aGGAGCccgGCGACgGGCUCGUGGCc -5' |
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30478 | 5' | -64.2 | NC_006548.1 | + | 23759 | 0.66 | 0.225337 |
Target: 5'- gUUCggCGGcGCCGCccacgUGCuuGAGCACCu- -3' miRNA: 3'- -AGGa-GCC-CGGCG-----ACGggCUCGUGGcc -5' |
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30478 | 5' | -64.2 | NC_006548.1 | + | 33032 | 0.66 | 0.219709 |
Target: 5'- gCCggcCGGccagcGCCGCUGCaCCGGGCGCg-- -3' miRNA: 3'- aGGa--GCC-----CGGCGACG-GGCUCGUGgcc -5' |
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30478 | 5' | -64.2 | NC_006548.1 | + | 37076 | 0.67 | 0.198385 |
Target: 5'- ---aUGGGCUGCUGCCgGuGCugCGc -3' miRNA: 3'- aggaGCCCGGCGACGGgCuCGugGCc -5' |
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30478 | 5' | -64.2 | NC_006548.1 | + | 29647 | 0.67 | 0.208812 |
Target: 5'- ---cUGGuGCCGCUGCCagcGGUGCCGGu -3' miRNA: 3'- aggaGCC-CGGCGACGGgc-UCGUGGCC- -5' |
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30478 | 5' | -64.2 | NC_006548.1 | + | 30785 | 0.67 | 0.193344 |
Target: 5'- -gCUCguagGGGCUGCugaUGCCCcGGUGCCGGu -3' miRNA: 3'- agGAG----CCCGGCG---ACGGGcUCGUGGCC- -5' |
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30478 | 5' | -64.2 | NC_006548.1 | + | 34481 | 0.67 | 0.208812 |
Target: 5'- aUCCg-GGGaaCGCgacaGCCCGAGUACCaGGa -3' miRNA: 3'- -AGGagCCCg-GCGa---CGGGCUCGUGG-CC- -5' |
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30478 | 5' | -64.2 | NC_006548.1 | + | 28843 | 0.67 | 0.214201 |
Target: 5'- aUCCa-GGGUCGCccaggcgGCgCGAGCACgGGc -3' miRNA: 3'- -AGGagCCCGGCGa------CGgGCUCGUGgCC- -5' |
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30478 | 5' | -64.2 | NC_006548.1 | + | 27937 | 0.68 | 0.1654 |
Target: 5'- gUUCUCGcuGCCGCcaagGCCCuGGGCACCa- -3' miRNA: 3'- -AGGAGCc-CGGCGa---CGGG-CUCGUGGcc -5' |
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30478 | 5' | -64.2 | NC_006548.1 | + | 23521 | 0.69 | 0.148838 |
Target: 5'- gCCUCGGGCCGgaUGaCCCGcugaccccgccaGGCGgUGGg -3' miRNA: 3'- aGGAGCCCGGCg-AC-GGGC------------UCGUgGCC- -5' |
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30478 | 5' | -64.2 | NC_006548.1 | + | 13301 | 0.69 | 0.152025 |
Target: 5'- cCCUCGGcGCCGguUUGCCCGccgcaggagcgcGCGCCGa -3' miRNA: 3'- aGGAGCC-CGGC--GACGGGCu-----------CGUGGCc -5' |
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30478 | 5' | -64.2 | NC_006548.1 | + | 13558 | 0.69 | 0.156922 |
Target: 5'- uUCCUCGGuGCC--UGCgCGGGCGgCGGc -3' miRNA: 3'- -AGGAGCC-CGGcgACGgGCUCGUgGCC- -5' |
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30478 | 5' | -64.2 | NC_006548.1 | + | 33137 | 0.7 | 0.113853 |
Target: 5'- aCCggggCuGGCCGgUGCUCGGGCAgCGGc -3' miRNA: 3'- aGGa---GcCCGGCgACGGGCUCGUgGCC- -5' |
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30478 | 5' | -64.2 | NC_006548.1 | + | 27518 | 0.71 | 0.094103 |
Target: 5'- gUCCagGGGCUGCUGUUCGccGGCaucGCCGGu -3' miRNA: 3'- -AGGagCCCGGCGACGGGC--UCG---UGGCC- -5' |
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30478 | 5' | -64.2 | NC_006548.1 | + | 33350 | 0.74 | 0.060472 |
Target: 5'- cUCCUCGcGGCCGgagguCUGCcaacggaagCCGuGCACCGGg -3' miRNA: 3'- -AGGAGC-CCGGC-----GACG---------GGCuCGUGGCC- -5' |
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30478 | 5' | -64.2 | NC_006548.1 | + | 15651 | 0.76 | 0.04321 |
Target: 5'- cCCUCGGGCUGCUGCCaguuaGcGGuCugCGGg -3' miRNA: 3'- aGGAGCCCGGCGACGGg----C-UC-GugGCC- -5' |
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30478 | 5' | -64.2 | NC_006548.1 | + | 13418 | 0.78 | 0.029098 |
Target: 5'- aCCggcggCGGGCCaggucGCUGUuuGAGCGCCGGc -3' miRNA: 3'- aGGa----GCCCGG-----CGACGggCUCGUGGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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