Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30480 | 3' | -58.1 | NC_006548.1 | + | 7069 | 0.66 | 0.395163 |
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Target: 5'- uGUCGGGCCGA-GUGGAucaacGcCGUGCu- -3' miRNA: 3'- -UAGCCCGGCUcCGCCUu----CaGCAUGuu -5' |
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| 30480 | 3' | -58.1 | NC_006548.1 | + | 13351 | 0.67 | 0.385942 |
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Target: 5'- -cCGGcGCCGAgGGCGGuguugAAGUCGcGCAc -3' miRNA: 3'- uaGCC-CGGCU-CCGCC-----UUCAGCaUGUu -5' |
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| 30480 | 3' | -58.1 | NC_006548.1 | + | 33347 | 0.67 | 0.367931 |
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Target: 5'- cUCGcGGCCgGAGGUcugccaacGGAAGcCGUGCAc -3' miRNA: 3'- uAGC-CCGG-CUCCG--------CCUUCaGCAUGUu -5' |
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| 30480 | 3' | -58.1 | NC_006548.1 | + | 17246 | 0.68 | 0.325486 |
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Target: 5'- -aCGGGCCG-GGCcGAGGUUGaACGAc -3' miRNA: 3'- uaGCCCGGCuCCGcCUUCAGCaUGUU- -5' |
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| 30480 | 3' | -58.1 | NC_006548.1 | + | 27851 | 0.68 | 0.309556 |
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Target: 5'- aGUgGuGGCCGAGGCGacgccuucGAAGaUCGUGCGc -3' miRNA: 3'- -UAgC-CCGGCUCCGC--------CUUC-AGCAUGUu -5' |
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| 30480 | 3' | -58.1 | NC_006548.1 | + | 1460 | 0.69 | 0.294227 |
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Target: 5'- cAUCGcGCCGAGGCGGA--UCGccgGCAGu -3' miRNA: 3'- -UAGCcCGGCUCCGCCUucAGCa--UGUU- -5' |
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| 30480 | 3' | -58.1 | NC_006548.1 | + | 25133 | 0.71 | 0.208823 |
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Target: 5'- gAUCGguGGCCGGGGCGGGcugGGUCuUGCGc -3' miRNA: 3'- -UAGC--CCGGCUCCGCCU---UCAGcAUGUu -5' |
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| 30480 | 3' | -58.1 | NC_006548.1 | + | 1675 | 0.72 | 0.16299 |
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Target: 5'- cAUUGGGCCGGGGCGGAgagAGuuaUCGgcuggGCAc -3' miRNA: 3'- -UAGCCCGGCUCCGCCU---UC---AGCa----UGUu -5' |
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| 30480 | 3' | -58.1 | NC_006548.1 | + | 33851 | 1.04 | 0.000624 |
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Target: 5'- gAUCGGGCCGAGGCGGAAGUCGUACAAg -3' miRNA: 3'- -UAGCCCGGCUCCGCCUUCAGCAUGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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