Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30482 | 3' | -53.6 | NC_006548.1 | + | 1935 | 0.66 | 0.721502 |
Target: 5'- uGGUGca-GCCGcgCGCGcUUGCCcGCGg -3' miRNA: 3'- -CCAUaggCGGUuaGCGC-AACGGaCGU- -5' |
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30482 | 3' | -53.6 | NC_006548.1 | + | 3413 | 0.66 | 0.710366 |
Target: 5'- cGGUAUCaaucugcagGUCGAUCGCGUcacCCUGUg -3' miRNA: 3'- -CCAUAGg--------CGGUUAGCGCAac-GGACGu -5' |
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30482 | 3' | -53.6 | NC_006548.1 | + | 25149 | 0.66 | 0.710366 |
Target: 5'- aGGUcAUCCGCCccugGAUCG-GUgGCCgggGCGg -3' miRNA: 3'- -CCA-UAGGCGG----UUAGCgCAaCGGa--CGU- -5' |
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30482 | 3' | -53.6 | NC_006548.1 | + | 32418 | 0.66 | 0.699147 |
Target: 5'- cGUcgCCGCCGuugccguUCGCGgcG-CUGCAg -3' miRNA: 3'- cCAuaGGCGGUu------AGCGCaaCgGACGU- -5' |
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30482 | 3' | -53.6 | NC_006548.1 | + | 29473 | 0.66 | 0.687857 |
Target: 5'- --cGUCgGCCAgcgAUCGCugaccGCCUGCAg -3' miRNA: 3'- ccaUAGgCGGU---UAGCGcaa--CGGACGU- -5' |
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30482 | 3' | -53.6 | NC_006548.1 | + | 29410 | 0.67 | 0.642241 |
Target: 5'- aGGcgGUCaGCCGAUCGCuggccgacGUUGCCgcGCAg -3' miRNA: 3'- -CCa-UAGgCGGUUAGCG--------CAACGGa-CGU- -5' |
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30482 | 3' | -53.6 | NC_006548.1 | + | 27012 | 0.67 | 0.642241 |
Target: 5'- --aGUCCGCCGAUCaGCagacgGCCcGCAu -3' miRNA: 3'- ccaUAGGCGGUUAG-CGcaa--CGGaCGU- -5' |
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30482 | 3' | -53.6 | NC_006548.1 | + | 24001 | 0.67 | 0.642241 |
Target: 5'- cGGUGUCCaGCCGAUCagg--GCCUuGCGu -3' miRNA: 3'- -CCAUAGG-CGGUUAGcgcaaCGGA-CGU- -5' |
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30482 | 3' | -53.6 | NC_006548.1 | + | 22290 | 0.67 | 0.635367 |
Target: 5'- gGGUGUCgGCCAggcgAUCGCGguagaugaaagacGCgUGCGg -3' miRNA: 3'- -CCAUAGgCGGU----UAGCGCaa-----------CGgACGU- -5' |
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30482 | 3' | -53.6 | NC_006548.1 | + | 15217 | 0.67 | 0.630783 |
Target: 5'- --cGUCCGCCGcgcUUGCcg-GCCUGCGc -3' miRNA: 3'- ccaUAGGCGGUu--AGCGcaaCGGACGU- -5' |
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30482 | 3' | -53.6 | NC_006548.1 | + | 32068 | 0.68 | 0.595318 |
Target: 5'- aGUAccUCgGCCGGUCGaagcgguCGUUGCCgGCAu -3' miRNA: 3'- cCAU--AGgCGGUUAGC-------GCAACGGaCGU- -5' |
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30482 | 3' | -53.6 | NC_006548.1 | + | 13215 | 0.68 | 0.562428 |
Target: 5'- gGGUGUCgGUCAGUUGCuGcUGuaCCUGCAg -3' miRNA: 3'- -CCAUAGgCGGUUAGCG-CaAC--GGACGU- -5' |
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30482 | 3' | -53.6 | NC_006548.1 | + | 12838 | 0.69 | 0.51794 |
Target: 5'- aGGUG-CCGgUAggaaGUCGCGacUGCCUGCGa -3' miRNA: 3'- -CCAUaGGCgGU----UAGCGCa-ACGGACGU- -5' |
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30482 | 3' | -53.6 | NC_006548.1 | + | 12313 | 0.69 | 0.50703 |
Target: 5'- cGGUA-CCGCUgagugGAUCGCcg-GCCUGUAu -3' miRNA: 3'- -CCAUaGGCGG-----UUAGCGcaaCGGACGU- -5' |
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30482 | 3' | -53.6 | NC_006548.1 | + | 29977 | 0.7 | 0.454086 |
Target: 5'- uGGcUAUgCCGCCcGUCGCG-UGCC-GCAc -3' miRNA: 3'- -CC-AUA-GGCGGuUAGCGCaACGGaCGU- -5' |
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30482 | 3' | -53.6 | NC_006548.1 | + | 34327 | 1.11 | 0.000702 |
Target: 5'- gGGUAUCCGCCAAUCGCGUUGCCUGCAc -3' miRNA: 3'- -CCAUAGGCGGUUAGCGCAACGGACGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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