Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30484 | 3' | -56.7 | NC_006548.1 | + | 23502 | 0.66 | 0.584081 |
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Target: 5'- -aUCgCCGAGGGCaaGUUCAG-CCUCGGg -3' miRNA: 3'- ccAG-GGCUCCUGc-UAGGUCaGGAGCU- -5' |
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| 30484 | 3' | -56.7 | NC_006548.1 | + | 21253 | 0.67 | 0.498689 |
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Target: 5'- -uUCCCGccgccGACGGUgCGGUCUUCGAc -3' miRNA: 3'- ccAGGGCuc---CUGCUAgGUCAGGAGCU- -5' |
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| 30484 | 3' | -56.7 | NC_006548.1 | + | 12400 | 0.68 | 0.468073 |
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Target: 5'- uGGUgCCGAGGGCGG-CCGGauacaggCCggCGAu -3' miRNA: 3'- -CCAgGGCUCCUGCUaGGUCa------GGa-GCU- -5' |
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| 30484 | 3' | -56.7 | NC_006548.1 | + | 23011 | 0.69 | 0.419312 |
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Target: 5'- cGGUCCuUGAGGccgGCGGcCCAGUCCacccCGAu -3' miRNA: 3'- -CCAGG-GCUCC---UGCUaGGUCAGGa---GCU- -5' |
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| 30484 | 3' | -56.7 | NC_006548.1 | + | 24583 | 0.7 | 0.373759 |
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Target: 5'- aGUCCuCGAGGGCGAUCUgaaAG-CCggCGAc -3' miRNA: 3'- cCAGG-GCUCCUGCUAGG---UCaGGa-GCU- -5' |
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| 30484 | 3' | -56.7 | NC_006548.1 | + | 34784 | 1.11 | 0.000414 |
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Target: 5'- cGGUCCCGAGGACGAUCCAGUCCUCGAu -3' miRNA: 3'- -CCAGGGCUCCUGCUAGGUCAGGAGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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