Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30484 | 5' | -55.5 | NC_006548.1 | + | 37295 | 0.66 | 0.596691 |
Target: 5'- gGCCGuuGAUCAaua---GGCCGGUGu -3' miRNA: 3'- gCGGCuuCUAGUgcaucaCCGGCCAC- -5' |
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30484 | 5' | -55.5 | NC_006548.1 | + | 20028 | 0.67 | 0.562247 |
Target: 5'- gCGCCGGaucguAGGUCGCGgcGaucuccugcaggaUGGCCucGGUGa -3' miRNA: 3'- -GCGGCU-----UCUAGUGCauC-------------ACCGG--CCAC- -5' |
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30484 | 5' | -55.5 | NC_006548.1 | + | 17619 | 0.67 | 0.541397 |
Target: 5'- gGCCGAGGggCugGaGGcagcucaccUGGCCGGa- -3' miRNA: 3'- gCGGCUUCuaGugCaUC---------ACCGGCCac -5' |
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30484 | 5' | -55.5 | NC_006548.1 | + | 2049 | 0.67 | 0.530527 |
Target: 5'- gCGCCGcAAGcucaaggCACGUgacgaAGUGGCCaGUGa -3' miRNA: 3'- -GCGGC-UUCua-----GUGCA-----UCACCGGcCAC- -5' |
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30484 | 5' | -55.5 | NC_006548.1 | + | 20279 | 0.67 | 0.519737 |
Target: 5'- uCGCCGAGGAUgACGgcGcccUGGCCa--- -3' miRNA: 3'- -GCGGCUUCUAgUGCauC---ACCGGccac -5' |
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30484 | 5' | -55.5 | NC_006548.1 | + | 23087 | 0.68 | 0.509035 |
Target: 5'- aGCgGGAGcggC-CGUAGUGGCCGuGUa -3' miRNA: 3'- gCGgCUUCua-GuGCAUCACCGGC-CAc -5' |
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30484 | 5' | -55.5 | NC_006548.1 | + | 20771 | 0.68 | 0.498426 |
Target: 5'- uCGCCGAAGGcCugGUcaaGGCCGGc- -3' miRNA: 3'- -GCGGCUUCUaGugCAucaCCGGCCac -5' |
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30484 | 5' | -55.5 | NC_006548.1 | + | 22593 | 0.69 | 0.40804 |
Target: 5'- cCGCCGgcGGcugugCGCcgGUAGUGGuuGGUGc -3' miRNA: 3'- -GCGGCuuCUa----GUG--CAUCACCggCCAC- -5' |
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30484 | 5' | -55.5 | NC_006548.1 | + | 33110 | 0.72 | 0.273025 |
Target: 5'- gGCCGGAGGUCAUGcccUcgacaacauaccggGGcUGGCCGGUGc -3' miRNA: 3'- gCGGCUUCUAGUGC---A--------------UC-ACCGGCCAC- -5' |
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30484 | 5' | -55.5 | NC_006548.1 | + | 31454 | 0.74 | 0.228268 |
Target: 5'- aCGUCG-AGAUCGCGgcaGGUcaguuccGGCCGGUGa -3' miRNA: 3'- -GCGGCuUCUAGUGCa--UCA-------CCGGCCAC- -5' |
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30484 | 5' | -55.5 | NC_006548.1 | + | 34749 | 1.07 | 0.000838 |
Target: 5'- uCGCCGAAGAUCACGUAGUGGCCGGUGc -3' miRNA: 3'- -GCGGCUUCUAGUGCAUCACCGGCCAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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