Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30485 | 3' | -52.4 | NC_006548.1 | + | 7282 | 0.66 | 0.727594 |
Target: 5'- uGCCGGUCgAGGUCAaccaggcgcUGGGUgaUGAcgGCa -3' miRNA: 3'- -CGGUCAGgUUCGGU---------ACCCGuaACU--UG- -5' |
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30485 | 3' | -52.4 | NC_006548.1 | + | 29612 | 0.66 | 0.74987 |
Target: 5'- aGCCAGUCUugcGGCCGcgaaUGGGCu------ -3' miRNA: 3'- -CGGUCAGGu--UCGGU----ACCCGuaacuug -5' |
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30485 | 3' | -52.4 | NC_006548.1 | + | 24360 | 0.66 | 0.760812 |
Target: 5'- gGCCGGUCUggGCCGgaaacgugccGGCcUUGAc- -3' miRNA: 3'- -CGGUCAGGuuCGGUac--------CCGuAACUug -5' |
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30485 | 3' | -52.4 | NC_006548.1 | + | 34946 | 1.11 | 0.000744 |
Target: 5'- uGCCAGUCCAAGCCAUGGGCAUUGAACc -3' miRNA: 3'- -CGGUCAGGUUCGGUACCCGUAACUUG- -5' |
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30485 | 3' | -52.4 | NC_006548.1 | + | 29176 | 0.7 | 0.520054 |
Target: 5'- gGCCAGUUCGcugacgagaccGGCCugcUGGGCGgccUGGAUa -3' miRNA: 3'- -CGGUCAGGU-----------UCGGu--ACCCGUa--ACUUG- -5' |
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30485 | 3' | -52.4 | NC_006548.1 | + | 35308 | 0.69 | 0.530166 |
Target: 5'- cGCCAGUCgAggauucaccgguuGGUCcgGGGCGccGAACa -3' miRNA: 3'- -CGGUCAGgU-------------UCGGuaCCCGUaaCUUG- -5' |
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30485 | 3' | -52.4 | NC_006548.1 | + | 31158 | 0.69 | 0.550595 |
Target: 5'- uGUCAGUCCuuccugauugcguaGAGCCAcGGGUugccgGAGCg -3' miRNA: 3'- -CGGUCAGG--------------UUCGGUaCCCGuaa--CUUG- -5' |
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30485 | 3' | -52.4 | NC_006548.1 | + | 16922 | 0.69 | 0.565498 |
Target: 5'- -gCGG-CCGAccGCCGUGGGCAUaccGAACu -3' miRNA: 3'- cgGUCaGGUU--CGGUACCCGUAa--CUUG- -5' |
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30485 | 3' | -52.4 | NC_006548.1 | + | 663 | 0.68 | 0.623584 |
Target: 5'- uGCCAG----GGCC-UGGGCAUcGAGCa -3' miRNA: 3'- -CGGUCagguUCGGuACCCGUAaCUUG- -5' |
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30485 | 3' | -52.4 | NC_006548.1 | + | 37526 | 0.68 | 0.635271 |
Target: 5'- cGCCAGcCC-AGCCGUGgcGGCAUgu-ACu -3' miRNA: 3'- -CGGUCaGGuUCGGUAC--CCGUAacuUG- -5' |
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30485 | 3' | -52.4 | NC_006548.1 | + | 20088 | 0.67 | 0.658622 |
Target: 5'- cCCAG-CCAuaGGCC--GGcGCAUUGAGCu -3' miRNA: 3'- cGGUCaGGU--UCGGuaCC-CGUAACUUG- -5' |
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30485 | 3' | -52.4 | NC_006548.1 | + | 17686 | 0.66 | 0.748768 |
Target: 5'- gGCCAGgugagcugccUCCAgccccucGGCCAuggccUGGGCGUauugaucaagacUGAGCu -3' miRNA: 3'- -CGGUC----------AGGU-------UCGGU-----ACCCGUA------------ACUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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