Results 41 - 45 of 45 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30485 | 5' | -56.4 | NC_006548.1 | + | 32963 | 0.71 | 0.316641 |
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Target: 5'- cGCGCCcGGUGCaGCGGcgcuGgCCGGCCGg -3' miRNA: 3'- -CGUGGcCUAUG-CGUCau--UgGGCCGGC- -5' |
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| 30485 | 5' | -56.4 | NC_006548.1 | + | 20864 | 0.73 | 0.238921 |
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Target: 5'- uCACCGGAgccuucugGCGCGGcAGCaggCGGCCGg -3' miRNA: 3'- cGUGGCCUa-------UGCGUCaUUGg--GCCGGC- -5' |
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| 30485 | 5' | -56.4 | NC_006548.1 | + | 6512 | 0.73 | 0.21488 |
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Target: 5'- cGCGCCaGGGcgGCGCGGguuacgGGCUCGGUCGa -3' miRNA: 3'- -CGUGG-CCUa-UGCGUCa-----UUGGGCCGGC- -5' |
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| 30485 | 5' | -56.4 | NC_006548.1 | + | 217 | 0.79 | 0.090411 |
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Target: 5'- aGCACCGGAgacaACGCcgaaccgaucAGUugggccuuuuuGACCCGGCCGa -3' miRNA: 3'- -CGUGGCCUa---UGCG----------UCA-----------UUGGGCCGGC- -5' |
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| 30485 | 5' | -56.4 | NC_006548.1 | + | 34911 | 1.12 | 0.000342 |
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Target: 5'- cGCACCGGAUACGCAGUAACCCGGCCGg -3' miRNA: 3'- -CGUGGCCUAUGCGUCAUUGGGCCGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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