Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30486 | 5' | -54.8 | NC_006548.1 | + | 7003 | 0.66 | 0.629722 |
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Target: 5'- -cCGauGUCGaUCAgCGcGAUCAGCCGCGc -3' miRNA: 3'- caGCauCAGC-AGU-GC-CUAGUCGGCGU- -5' |
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| 30486 | 5' | -54.8 | NC_006548.1 | + | 14503 | 0.67 | 0.562205 |
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Target: 5'- cGUCcaGGUCGUCgccgccugggcACGGAUCagcuuGGCCGCc -3' miRNA: 3'- -CAGcaUCAGCAG-----------UGCCUAG-----UCGGCGu -5' |
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| 30486 | 5' | -54.8 | NC_006548.1 | + | 6979 | 0.68 | 0.507474 |
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Target: 5'- --gGUGcGUCGUCgacagacuccgcACGGAUCaAGCCGCu -3' miRNA: 3'- cagCAU-CAGCAG------------UGCCUAG-UCGGCGu -5' |
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| 30486 | 5' | -54.8 | NC_006548.1 | + | 28293 | 0.68 | 0.507474 |
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Target: 5'- cGUCGUcaacgucaccaAGuUCGUCGCGGAggagauugCGGCgCGCGu -3' miRNA: 3'- -CAGCA-----------UC-AGCAGUGCCUa-------GUCG-GCGU- -5' |
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| 30486 | 5' | -54.8 | NC_006548.1 | + | 17962 | 0.68 | 0.507474 |
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Target: 5'- cGUCGUAGUCcUUGCGGAacuccgccagggUCAuGCCGUc -3' miRNA: 3'- -CAGCAUCAGcAGUGCCU------------AGU-CGGCGu -5' |
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| 30486 | 5' | -54.8 | NC_006548.1 | + | 35764 | 1.09 | 0.000718 |
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Target: 5'- cGUCGUAGUCGUCACGGAUCAGCCGCAg -3' miRNA: 3'- -CAGCAUCAGCAGUGCCUAGUCGGCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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