Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30487 | 5' | -55.2 | NC_006548.1 | + | 17974 | 0.66 | 0.623092 |
Target: 5'- gGCGCG-AGUgaucuacgagacgaAUCUGCGcaccagcuaugcGGCUGGACg -3' miRNA: 3'- -CGCGCaUCAag------------UAGACGU------------CCGGCCUG- -5' |
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30487 | 5' | -55.2 | NC_006548.1 | + | 7654 | 0.66 | 0.620822 |
Target: 5'- gGCGCGUcuucuccAGUUCGagcuugguguUCUGCAGGUucuCGGu- -3' miRNA: 3'- -CGCGCA-------UCAAGU----------AGACGUCCG---GCCug -5' |
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30487 | 5' | -55.2 | NC_006548.1 | + | 2336 | 0.66 | 0.610615 |
Target: 5'- aGCGCuucGUuccuGUUCGUCggcugcgcgGCGGcGCCGGAg -3' miRNA: 3'- -CGCG---CAu---CAAGUAGa--------CGUC-CGGCCUg -5' |
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30487 | 5' | -55.2 | NC_006548.1 | + | 24024 | 0.66 | 0.599292 |
Target: 5'- uCGCG-AGcgC--CUGcCAGGCCGGGCg -3' miRNA: 3'- cGCGCaUCaaGuaGAC-GUCCGGCCUG- -5' |
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30487 | 5' | -55.2 | NC_006548.1 | + | 22241 | 0.66 | 0.599292 |
Target: 5'- gGCGCGaaucaGGUUCGgguUCUGCcccGGCCGaGCg -3' miRNA: 3'- -CGCGCa----UCAAGU---AGACGu--CCGGCcUG- -5' |
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30487 | 5' | -55.2 | NC_006548.1 | + | 32727 | 0.66 | 0.588 |
Target: 5'- aGCGCGUGcUccucgaccUCAgccUUGCAGGCUGGGg -3' miRNA: 3'- -CGCGCAUcA--------AGUa--GACGUCCGGCCUg -5' |
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30487 | 5' | -55.2 | NC_006548.1 | + | 10391 | 0.66 | 0.57787 |
Target: 5'- cGCGCGUgaacaucgaGGUgacagguagcaacaaCAUagUUGCAGGCCGGGa -3' miRNA: 3'- -CGCGCA---------UCAa--------------GUA--GACGUCCGGCCUg -5' |
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30487 | 5' | -55.2 | NC_006548.1 | + | 36397 | 0.66 | 0.576747 |
Target: 5'- cCGCGUGGg--AUCUGCAcGGUucgaagagCGGACg -3' miRNA: 3'- cGCGCAUCaagUAGACGU-CCG--------GCCUG- -5' |
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30487 | 5' | -55.2 | NC_006548.1 | + | 6852 | 0.67 | 0.565542 |
Target: 5'- -aGaCGUGGUUCGgaUGCAGGCUGcGCa -3' miRNA: 3'- cgC-GCAUCAAGUagACGUCCGGCcUG- -5' |
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30487 | 5' | -55.2 | NC_006548.1 | + | 32568 | 0.67 | 0.510541 |
Target: 5'- cCGCGUGGc-CGUUUGC--GCCGGACu -3' miRNA: 3'- cGCGCAUCaaGUAGACGucCGGCCUG- -5' |
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30487 | 5' | -55.2 | NC_006548.1 | + | 28149 | 0.68 | 0.478621 |
Target: 5'- -gGCGguugAGUUCGgcCUGCAGGCCGc-- -3' miRNA: 3'- cgCGCa---UCAAGUa-GACGUCCGGCcug -5' |
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30487 | 5' | -55.2 | NC_006548.1 | + | 18440 | 0.7 | 0.389414 |
Target: 5'- aGCgGCGUGG-UCuUCUGCAGGgcgccCUGGACc -3' miRNA: 3'- -CG-CGCAUCaAGuAGACGUCC-----GGCCUG- -5' |
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30487 | 5' | -55.2 | NC_006548.1 | + | 36428 | 1.12 | 0.000339 |
Target: 5'- aGCGCGUAGUUCAUCUGCAGGCCGGACg -3' miRNA: 3'- -CGCGCAUCAAGUAGACGUCCGGCCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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