Results 1 - 20 of 21 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30488 | 3' | -53.7 | NC_006548.1 | + | 17577 | 0.66 | 0.742907 |
Target: 5'- gCCugCUCGAUGAGcGUgCGcaguuGGGUCGu- -3' miRNA: 3'- gGGugGAGCUGCUC-CA-GU-----UCCAGUug -5' |
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30488 | 3' | -53.7 | NC_006548.1 | + | 869 | 0.66 | 0.742907 |
Target: 5'- gCCaguuGCC-CGcCGAGGUCcAGGUCAu- -3' miRNA: 3'- -GGg---UGGaGCuGCUCCAGuUCCAGUug -5' |
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30488 | 3' | -53.7 | NC_006548.1 | + | 7167 | 0.66 | 0.742907 |
Target: 5'- gCCGCCUggggcgUGACGAGGcCcuGGugaUCAGCa -3' miRNA: 3'- gGGUGGA------GCUGCUCCaGuuCC---AGUUG- -5' |
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30488 | 3' | -53.7 | NC_006548.1 | + | 26751 | 0.66 | 0.742907 |
Target: 5'- gCCACCUcCGACcguguAGGUgAugucGGUCGACu -3' miRNA: 3'- gGGUGGA-GCUGc----UCCAgUu---CCAGUUG- -5' |
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30488 | 3' | -53.7 | NC_006548.1 | + | 8414 | 0.66 | 0.72121 |
Target: 5'- gCCgGCCUCGAUGAGGgucugCAuguagcGGGUgAu- -3' miRNA: 3'- -GGgUGGAGCUGCUCCa----GU------UCCAgUug -5' |
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30488 | 3' | -53.7 | NC_006548.1 | + | 792 | 0.66 | 0.732112 |
Target: 5'- aUCGCCaagcagauguUCGGCGuGGcCAAGGUCGc- -3' miRNA: 3'- gGGUGG----------AGCUGCuCCaGUUCCAGUug -5' |
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30488 | 3' | -53.7 | NC_006548.1 | + | 32800 | 0.66 | 0.732112 |
Target: 5'- cCCCAgCCUgcaaGGCuGAGGUCGAGG--AGCa -3' miRNA: 3'- -GGGU-GGAg---CUG-CUCCAGUUCCagUUG- -5' |
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30488 | 3' | -53.7 | NC_006548.1 | + | 17210 | 0.66 | 0.72121 |
Target: 5'- aUCCGCgUCGGCcuucaccAGGUCAAGG-CGAa -3' miRNA: 3'- -GGGUGgAGCUGc------UCCAGUUCCaGUUg -5' |
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30488 | 3' | -53.7 | NC_006548.1 | + | 31015 | 0.66 | 0.7069 |
Target: 5'- gCCCAggUUUCGGCGAcccucgguuucgcgGGUCAcgAGGcUCAACg -3' miRNA: 3'- -GGGU--GGAGCUGCU--------------CCAGU--UCC-AGUUG- -5' |
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30488 | 3' | -53.7 | NC_006548.1 | + | 7259 | 0.67 | 0.68352 |
Target: 5'- gCCC-CUgacCGGCGcucuguagcugccGGUCGAGGUCAACc -3' miRNA: 3'- -GGGuGGa--GCUGCu------------CCAGUUCCAGUUG- -5' |
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30488 | 3' | -53.7 | NC_006548.1 | + | 30297 | 0.67 | 0.642968 |
Target: 5'- gCCCAgCCgCGACcAGGUCuGGGUUcGCg -3' miRNA: 3'- -GGGU-GGaGCUGcUCCAGuUCCAGuUG- -5' |
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30488 | 3' | -53.7 | NC_006548.1 | + | 7348 | 0.67 | 0.658784 |
Target: 5'- aCCCagcgccugguugACCUCGAcCGGcagcuacagagcgccGGUCAGGGgCAGCg -3' miRNA: 3'- -GGG------------UGGAGCU-GCU---------------CCAGUUCCaGUUG- -5' |
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30488 | 3' | -53.7 | NC_006548.1 | + | 15930 | 0.68 | 0.620347 |
Target: 5'- uCCUuuaCUCG-CGGGGUgAAGGUCAc- -3' miRNA: 3'- -GGGug-GAGCuGCUCCAgUUCCAGUug -5' |
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30488 | 3' | -53.7 | NC_006548.1 | + | 36519 | 0.68 | 0.597769 |
Target: 5'- aCCUugACCUCGuCGAGGU--GGGcUCGGCc -3' miRNA: 3'- -GGG--UGGAGCuGCUCCAguUCC-AGUUG- -5' |
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30488 | 3' | -53.7 | NC_006548.1 | + | 633 | 0.68 | 0.579791 |
Target: 5'- aUCCACUUCGGCGAGG---AGGuaccguuucuccugcUCGACg -3' miRNA: 3'- -GGGUGGAGCUGCUCCaguUCC---------------AGUUG- -5' |
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30488 | 3' | -53.7 | NC_006548.1 | + | 17141 | 0.69 | 0.564156 |
Target: 5'- uUCGCCUUGACcuGGUgAAGGcCGACg -3' miRNA: 3'- gGGUGGAGCUGcuCCAgUUCCaGUUG- -5' |
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30488 | 3' | -53.7 | NC_006548.1 | + | 18284 | 0.7 | 0.47762 |
Target: 5'- aCCUACCggGGCGAGGuugUCGAGGUUc-- -3' miRNA: 3'- -GGGUGGagCUGCUCC---AGUUCCAGuug -5' |
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30488 | 3' | -53.7 | NC_006548.1 | + | 7637 | 0.74 | 0.273945 |
Target: 5'- gUCACCUCcauggccuAUGAGGUCGAGGUCGGg -3' miRNA: 3'- gGGUGGAGc-------UGCUCCAGUUCCAGUUg -5' |
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30488 | 3' | -53.7 | NC_006548.1 | + | 3627 | 0.75 | 0.25317 |
Target: 5'- aCCGCCUCGGCGcucAGcGUCAuGGUCGGg -3' miRNA: 3'- gGGUGGAGCUGC---UC-CAGUuCCAGUUg -5' |
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30488 | 3' | -53.7 | NC_006548.1 | + | 23599 | 0.82 | 0.091982 |
Target: 5'- cCCCACCgccUGGCGGGGUCAgcGGGUCAu- -3' miRNA: 3'- -GGGUGGa--GCUGCUCCAGU--UCCAGUug -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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