Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30488 | 5' | -57.3 | NC_006548.1 | + | 1507 | 0.66 | 0.476627 |
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Target: 5'- cGGCGcGAUGCUGACGUaaagcGG-CAGGa -3' miRNA: 3'- uUCGCaCUGCGACUGCGa----CCaGUCCg -5' |
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| 30488 | 5' | -57.3 | NC_006548.1 | + | 5605 | 0.68 | 0.370106 |
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Target: 5'- uGGCGaUGGCGUcGA-GCUGGUCuuccGGCa -3' miRNA: 3'- uUCGC-ACUGCGaCUgCGACCAGu---CCG- -5' |
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| 30488 | 5' | -57.3 | NC_006548.1 | + | 13020 | 0.68 | 0.34401 |
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Target: 5'- cGAGgGUGACGCUG-CGCUGcUCAaccuGCa -3' miRNA: 3'- -UUCgCACUGCGACuGCGACcAGUc---CG- -5' |
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| 30488 | 5' | -57.3 | NC_006548.1 | + | 14667 | 0.71 | 0.240079 |
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Target: 5'- cAGCGUGACGCUGgaGCGC--GUCAGcGUc -3' miRNA: 3'- uUCGCACUGCGAC--UGCGacCAGUC-CG- -5' |
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| 30488 | 5' | -57.3 | NC_006548.1 | + | 28522 | 0.71 | 0.227576 |
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Target: 5'- cAGCGUGACGCaguccugcucGGCGCcGGcCAGGUc -3' miRNA: 3'- uUCGCACUGCGa---------CUGCGaCCaGUCCG- -5' |
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| 30488 | 5' | -57.3 | NC_006548.1 | + | 20723 | 0.71 | 0.227576 |
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Target: 5'- cGAGC-UGGCGUUGGCGCUccuGGagcgcagcuucgUCAGGCa -3' miRNA: 3'- -UUCGcACUGCGACUGCGA---CC------------AGUCCG- -5' |
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| 30488 | 5' | -57.3 | NC_006548.1 | + | 29873 | 0.73 | 0.163705 |
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Target: 5'- cAAGCGUGguugggcuGCGCUGcAUGaCUGGUCcGGCg -3' miRNA: 3'- -UUCGCAC--------UGCGAC-UGC-GACCAGuCCG- -5' |
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| 30488 | 5' | -57.3 | NC_006548.1 | + | 36543 | 1.08 | 0.00039 |
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Target: 5'- cAAGCGUGACGCUGACGCUGGUCAGGCc -3' miRNA: 3'- -UUCGCACUGCGACUGCGACCAGUCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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