Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30489 | 3' | -55.5 | NC_006548.1 | + | 18034 | 0.66 | 0.58949 |
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Target: 5'- -gCUGCAGGCgGUCaaggGCGAGCGaccguuCUGGAu -3' miRNA: 3'- gaGACGUCUGgCAG----CGCUUGC------GACUU- -5' |
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| 30489 | 3' | -55.5 | NC_006548.1 | + | 32368 | 0.66 | 0.578191 |
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Target: 5'- gUC-GCAGAUCGaUCGCGAggACGCcGAu -3' miRNA: 3'- gAGaCGUCUGGC-AGCGCU--UGCGaCUu -5' |
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| 30489 | 3' | -55.5 | NC_006548.1 | + | 29405 | 0.67 | 0.490214 |
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Target: 5'- -cCUGCAGGCgGUCaGCcGAuCGCUGGc -3' miRNA: 3'- gaGACGUCUGgCAG-CG-CUuGCGACUu -5' |
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| 30489 | 3' | -55.5 | NC_006548.1 | + | 19616 | 0.67 | 0.490214 |
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Target: 5'- aUCUGCAGcGCgCGcucCGCGAACGCgccUGAAa -3' miRNA: 3'- gAGACGUC-UG-GCa--GCGCUUGCG---ACUU- -5' |
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| 30489 | 3' | -55.5 | NC_006548.1 | + | 32295 | 0.69 | 0.408976 |
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Target: 5'- aUCUGgGGAaCGUCGUGAGCGUgcagGAAc -3' miRNA: 3'- gAGACgUCUgGCAGCGCUUGCGa---CUU- -5' |
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| 30489 | 3' | -55.5 | NC_006548.1 | + | 2769 | 0.69 | 0.39942 |
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Target: 5'- -cCUGCAGAUCGaUGaCGAACGUUGGc -3' miRNA: 3'- gaGACGUCUGGCaGC-GCUUGCGACUu -5' |
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| 30489 | 3' | -55.5 | NC_006548.1 | + | 36506 | 0.69 | 0.390009 |
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Target: 5'- aUCUGCugAGAUCGUCGCGAccGgGCaGAAg -3' miRNA: 3'- gAGACG--UCUGGCAGCGCU--UgCGaCUU- -5' |
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| 30489 | 3' | -55.5 | NC_006548.1 | + | 25273 | 0.72 | 0.260037 |
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Target: 5'- --gUGCAGAUCGUCGagGAACgGCUGAAu -3' miRNA: 3'- gagACGUCUGGCAGCg-CUUG-CGACUU- -5' |
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| 30489 | 3' | -55.5 | NC_006548.1 | + | 37172 | 1.06 | 0.000837 |
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Target: 5'- aCUCUGCAGACCGUCGCGAACGCUGAAc -3' miRNA: 3'- -GAGACGUCUGGCAGCGCUUGCGACUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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