miRNA: 3'- cUCg--CAAAGGUGGuCCCGga---ACUCCU- -5'
miRNA: 3'- cUCGCAA--AGGUGGuc---CCGGaACUCCU- -5'
miRNA: 3'- -CUCGCaa-AGGU-----GGUCCCGGAACuccu -5'
miRNA: 3'- cUCGCaaaggUGGUCCCGGaaCUCCU- -5'
miRNA: 3'- -CUCGCAAAGGUGGUCCCGGAACUCCU- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30491 | 3' | -56.1 | NC_006548.1 | + | 16445 | 0.66 | 0.534249 |
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Target: 5'- cAGacaGUUUUCACCuGGGCaucgcUGAGGAu -3' miRNA: 3'- cUCg--CAAAGGUGGuCCCGga---ACUCCU- -5' |
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| 30491 | 3' | -56.1 | NC_006548.1 | + | 9846 | 0.66 | 0.523316 |
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Target: 5'- aAGCGUUgcUUCACCuggucGGCCgUGAGGGc -3' miRNA: 3'- cUCGCAA--AGGUGGuc---CCGGaACUCCU- -5' |
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| 30491 | 3' | -56.1 | NC_006548.1 | + | 24005 | 0.66 | 0.512469 |
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Target: 5'- cGGGCGgugUCCAgccgaUCAGGGCCUUGc--- -3' miRNA: 3'- -CUCGCaa-AGGU-----GGUCCCGGAACuccu -5' |
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| 30491 | 3' | -56.1 | NC_006548.1 | + | 15816 | 0.67 | 0.491063 |
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Target: 5'- cAGCGcuggaACCAGGGCCa-GAGGAu -3' miRNA: 3'- cUCGCaaaggUGGUCCCGGaaCUCCU- -5' |
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| 30491 | 3' | -56.1 | NC_006548.1 | + | 518 | 1.08 | 0.000512 |
|
Target: 5'- gGAGCGUUUCCACCAGGGCCUUGAGGAu -3' miRNA: 3'- -CUCGCAAAGGUGGUCCCGGAACUCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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