miRNA: 3'- -CGUC---UCGgC-UAGGUgAAGCCGCU--CCU- -5'
miRNA: 3'- -CGUCU---CGGCUAGGUgAaGC------CGCUCCU- -5'
miRNA: 3'- -CGUCUCGGCUAGGUGAAGCCGCUCCU- -5'
Results 1 - 3 of 3 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30492 | 3' | -56.3 | NC_006548.1 | + | 36271 | 0.67 | 0.507908 |
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Target: 5'- uGCGGucgGGCgGgGUCCAgUUCGGCGGcuGGAc -3' miRNA: 3'- -CGUC---UCGgC-UAGGUgAAGCCGCU--CCU- -5' |
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| 30492 | 3' | -56.3 | NC_006548.1 | + | 13758 | 0.71 | 0.289525 |
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Target: 5'- gGCGGAgcuGCCGAUCCAgUaCGuccgccGCGAGGAc -3' miRNA: 3'- -CGUCU---CGGCUAGGUgAaGC------CGCUCCU- -5' |
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| 30492 | 3' | -56.3 | NC_006548.1 | + | 623 | 1.11 | 0.000388 |
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Target: 5'- gGCAGAGCCGAUCCACUUCGGCGAGGAg -3' miRNA: 3'- -CGUCUCGGCUAGGUGAAGCCGCUCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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