miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
30493 5' -55.3 NC_006548.1 + 17192 0.66 0.56694
Target:  5'- aCCUCGGCccGGCccgUUGGCUGAccgAGUg- -3'
miRNA:   3'- -GGAGCCGc-CCGuu-GACCGACU---UCAau -5'
30493 5' -55.3 NC_006548.1 + 27775 0.66 0.56694
Target:  5'- aCCa-GGCGGGaauaGGCuUGGCUGguGUUGa -3'
miRNA:   3'- -GGagCCGCCCg---UUG-ACCGACuuCAAU- -5'
30493 5' -55.3 NC_006548.1 + 3630 0.67 0.533559
Target:  5'- gCCUCGGCGcuCAGCgucauggucggGGCUGAAGc-- -3'
miRNA:   3'- -GGAGCCGCccGUUGa----------CCGACUUCaau -5'
30493 5' -55.3 NC_006548.1 + 778 0.68 0.47963
Target:  5'- gUUCGGCGuGGCcaaggucGCaUGGCUGAAGa-- -3'
miRNA:   3'- gGAGCCGC-CCGu------UG-ACCGACUUCaau -5'
30493 5' -55.3 NC_006548.1 + 17664 0.69 0.39942
Target:  5'- cCCUCGGCcauggccuGGGCGuauugaucaagACUGaGCUGggGa-- -3'
miRNA:   3'- -GGAGCCG--------CCCGU-----------UGAC-CGACuuCaau -5'
30493 5' -55.3 NC_006548.1 + 13367 0.71 0.328315
Target:  5'- uCCUgCGGCGGGCAAaccGGCgccGAGGg-- -3'
miRNA:   3'- -GGA-GCCGCCCGUUga-CCGa--CUUCaau -5'
30493 5' -55.3 NC_006548.1 + 814 1.09 0.00056
Target:  5'- aCCUCGGCGGGCAACUGGCUGAAGUUAu -3'
miRNA:   3'- -GGAGCCGCCCGUUGACCGACUUCAAU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.