Results 1 - 7 of 7 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30493 | 5' | -55.3 | NC_006548.1 | + | 17192 | 0.66 | 0.56694 |
Target: 5'- aCCUCGGCccGGCccgUUGGCUGAccgAGUg- -3' miRNA: 3'- -GGAGCCGc-CCGuu-GACCGACU---UCAau -5' |
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30493 | 5' | -55.3 | NC_006548.1 | + | 27775 | 0.66 | 0.56694 |
Target: 5'- aCCa-GGCGGGaauaGGCuUGGCUGguGUUGa -3' miRNA: 3'- -GGagCCGCCCg---UUG-ACCGACuuCAAU- -5' |
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30493 | 5' | -55.3 | NC_006548.1 | + | 3630 | 0.67 | 0.533559 |
Target: 5'- gCCUCGGCGcuCAGCgucauggucggGGCUGAAGc-- -3' miRNA: 3'- -GGAGCCGCccGUUGa----------CCGACUUCaau -5' |
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30493 | 5' | -55.3 | NC_006548.1 | + | 778 | 0.68 | 0.47963 |
Target: 5'- gUUCGGCGuGGCcaaggucGCaUGGCUGAAGa-- -3' miRNA: 3'- gGAGCCGC-CCGu------UG-ACCGACUUCaau -5' |
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30493 | 5' | -55.3 | NC_006548.1 | + | 17664 | 0.69 | 0.39942 |
Target: 5'- cCCUCGGCcauggccuGGGCGuauugaucaagACUGaGCUGggGa-- -3' miRNA: 3'- -GGAGCCG--------CCCGU-----------UGAC-CGACuuCaau -5' |
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30493 | 5' | -55.3 | NC_006548.1 | + | 13367 | 0.71 | 0.328315 |
Target: 5'- uCCUgCGGCGGGCAAaccGGCgccGAGGg-- -3' miRNA: 3'- -GGA-GCCGCCCGUUga-CCGa--CUUCaau -5' |
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30493 | 5' | -55.3 | NC_006548.1 | + | 814 | 1.09 | 0.00056 |
Target: 5'- aCCUCGGCGGGCAACUGGCUGAAGUUAu -3' miRNA: 3'- -GGAGCCGCCCGUUGACCGACUUCAAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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