Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
30496 | 3' | -53.3 | NC_006548.1 | + | 36832 | 0.66 | 0.70102 |
Target: 5'- uGGccGGGUGCuGGUG-UguGGUGUG-GCg -3' miRNA: 3'- -CC--UCCACGuCUACuAguCCACGCaCG- -5' |
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30496 | 3' | -53.3 | NC_006548.1 | + | 6090 | 0.67 | 0.632741 |
Target: 5'- aGGGGuUGCGGGUGAUCAgcacacGGU-CGUGg -3' miRNA: 3'- cCUCC-ACGUCUACUAGU------CCAcGCACg -5' |
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30496 | 3' | -53.3 | NC_006548.1 | + | 3318 | 0.67 | 0.632741 |
Target: 5'- uGAGcUGCAGAgcccgcggUGAuuUCGGGguacgGCGUGCu -3' miRNA: 3'- cCUCcACGUCU--------ACU--AGUCCa----CGCACG- -5' |
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30496 | 3' | -53.3 | NC_006548.1 | + | 11421 | 0.69 | 0.557653 |
Target: 5'- uGGGGG-GCGGAUGGggcugcuaauggagCGGGUGUGUu- -3' miRNA: 3'- -CCUCCaCGUCUACUa-------------GUCCACGCAcg -5' |
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30496 | 3' | -53.3 | NC_006548.1 | + | 29663 | 0.7 | 0.466336 |
Target: 5'- --cGGUGCcGGUGcggCAGGUGCG-GCu -3' miRNA: 3'- ccuCCACGuCUACua-GUCCACGCaCG- -5' |
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30496 | 3' | -53.3 | NC_006548.1 | + | 2307 | 1.14 | 0.000473 |
Target: 5'- aGGAGGUGCAGAUGAUCAGGUGCGUGCc -3' miRNA: 3'- -CCUCCACGUCUACUAGUCCACGCACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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