Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30498 | 5' | -57.1 | NC_006548.1 | + | 23658 | 0.7 | 0.335121 |
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Target: 5'- cCUGGUGAUCGAGCGCUuGCGUg--- -3' miRNA: 3'- cGGCCGCUAGCUCGUGGcUGCAgacu -5' |
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| 30498 | 5' | -57.1 | NC_006548.1 | + | 37547 | 0.7 | 0.319138 |
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Target: 5'- cGCCGGCGGUUGGGCugaGugGaCUGu -3' miRNA: 3'- -CGGCCGCUAGCUCGuggCugCaGACu -5' |
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| 30498 | 5' | -57.1 | NC_006548.1 | + | 22781 | 0.71 | 0.288908 |
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Target: 5'- gGCCGGCa--CGAGCACCuucuGCGcCUGAa -3' miRNA: 3'- -CGGCCGcuaGCUCGUGGc---UGCaGACU- -5' |
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| 30498 | 5' | -57.1 | NC_006548.1 | + | 12636 | 0.72 | 0.247851 |
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Target: 5'- aGuuGGCGGcCGAGCGcCCGGCGU-UGAa -3' miRNA: 3'- -CggCCGCUaGCUCGU-GGCUGCAgACU- -5' |
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| 30498 | 5' | -57.1 | NC_006548.1 | + | 20820 | 0.73 | 0.20075 |
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Target: 5'- uCCGGUGAUCGAGCuGCUGGugaGUCUGc -3' miRNA: 3'- cGGCCGCUAGCUCG-UGGCUg--CAGACu -5' |
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| 30498 | 5' | -57.1 | NC_006548.1 | + | 22686 | 0.75 | 0.148819 |
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Target: 5'- cGCUGGCGAUCGAGCAgCu-CGUCg-- -3' miRNA: 3'- -CGGCCGCUAGCUCGUgGcuGCAGacu -5' |
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| 30498 | 5' | -57.1 | NC_006548.1 | + | 4198 | 1.11 | 0.000377 |
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Target: 5'- aGCCGGCGAUCGAGCACCGACGUCUGAu -3' miRNA: 3'- -CGGCCGCUAGCUCGUGGCUGCAGACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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