Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 3050 | 5' | -53.8 | NC_001493.1 | + | 37778 | 0.7 | 0.833329 |
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Target: 5'- aCUUCGcuauacGAGGuUCCAcUCACGG-GGUUCg -3' miRNA: 3'- -GAAGCu-----CUCC-AGGU-AGUGCCaCCAGG- -5' |
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| 3050 | 5' | -53.8 | NC_001493.1 | + | 106199 | 0.72 | 0.70141 |
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Target: 5'- -cUUGAGuAGGUCgcUCGCGGUGGcCCu -3' miRNA: 3'- gaAGCUC-UCCAGguAGUGCCACCaGG- -5' |
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| 3050 | 5' | -53.8 | NC_001493.1 | + | 108698 | 0.73 | 0.639781 |
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Target: 5'- -cUCGAGccucGGGUCCAUCGCaGGcgaUGGUCg -3' miRNA: 3'- gaAGCUC----UCCAGGUAGUG-CC---ACCAGg -5' |
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| 3050 | 5' | -53.8 | NC_001493.1 | + | 87232 | 0.76 | 0.511512 |
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Target: 5'- -cUCGGGGGGUCCAaCGCGGgggggauaucggagaUGGUCg -3' miRNA: 3'- gaAGCUCUCCAGGUaGUGCC---------------ACCAGg -5' |
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| 3050 | 5' | -53.8 | NC_001493.1 | + | 12994 | 0.76 | 0.497815 |
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Target: 5'- gUUCGAGAaucgGGUCUcuGUCACGGggccggacgGGUCCg -3' miRNA: 3'- gAAGCUCU----CCAGG--UAGUGCCa--------CCAGG- -5' |
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| 3050 | 5' | -53.8 | NC_001493.1 | + | 128548 | 0.76 | 0.497815 |
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Target: 5'- gUUCGAGAaucgGGUCUcuGUCACGGggccggacgGGUCCg -3' miRNA: 3'- gAAGCUCU----CCAGG--UAGUGCCa--------CCAGG- -5' |
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| 3050 | 5' | -53.8 | NC_001493.1 | + | 29738 | 1.11 | 0.00329 |
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Target: 5'- cCUUCGAGAGGUCCAUCACGGUGGUCCc -3' miRNA: 3'- -GAAGCUCUCCAGGUAGUGCCACCAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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