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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30500 | 3' | -55.2 | NC_006548.1 | + | 20753 | 0.66 | 0.599292 |
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Target: 5'- --cGCGGGUGGcCAGCAguugcuCGCGcUCGUc -3' miRNA: 3'- caaCGCCCACUaGUCGU------GUGCcAGCA- -5' |
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| 30500 | 3' | -55.2 | NC_006548.1 | + | 22330 | 0.67 | 0.554395 |
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Target: 5'- -cUGCGcGGUGGcCAGCGCGucCGuGUCGg -3' miRNA: 3'- caACGC-CCACUaGUCGUGU--GC-CAGCa -5' |
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| 30500 | 3' | -55.2 | NC_006548.1 | + | 16731 | 0.67 | 0.554395 |
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Target: 5'- uGUUGgGGGUGAgcaGGCGCAggccgccguccUGGUCa- -3' miRNA: 3'- -CAACgCCCACUag-UCGUGU-----------GCCAGca -5' |
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| 30500 | 3' | -55.2 | NC_006548.1 | + | 22296 | 0.7 | 0.362189 |
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Target: 5'- cGUUGCGGGUG-UCGGCcaggcgauCGCGGUa-- -3' miRNA: 3'- -CAACGCCCACuAGUCGu-------GUGCCAgca -5' |
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| 30500 | 3' | -55.2 | NC_006548.1 | + | 27870 | 0.71 | 0.311815 |
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Target: 5'- --gGCGGGUGAggugCAGCGCAgUGGUgGc -3' miRNA: 3'- caaCGCCCACUa---GUCGUGU-GCCAgCa -5' |
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| 30500 | 3' | -55.2 | NC_006548.1 | + | 33719 | 0.76 | 0.141615 |
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Target: 5'- --cGUGGGUGAUCuGCACGCuGUCGc -3' miRNA: 3'- caaCGCCCACUAGuCGUGUGcCAGCa -5' |
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| 30500 | 3' | -55.2 | NC_006548.1 | + | 6093 | 1.07 | 0.00078 |
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Target: 5'- gGUUGCGGGUGAUCAGCACACGGUCGUg -3' miRNA: 3'- -CAACGCCCACUAGUCGUGUGCCAGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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