Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30501 | 5' | -55.8 | NC_006548.1 | + | 13871 | 0.66 | 0.570001 |
Target: 5'- cGUCGaGCUUCGCCucgaucACGCUCUgGCcgcGGa -3' miRNA: 3'- -CAGC-CGAGGUGG------UGCGAGAaCGua-CC- -5' |
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30501 | 5' | -55.8 | NC_006548.1 | + | 27471 | 0.66 | 0.558893 |
Target: 5'- --aGGcCUCgACCcugGCGCUCaaugGCAUGGg -3' miRNA: 3'- cagCC-GAGgUGG---UGCGAGaa--CGUACC- -5' |
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30501 | 5' | -55.8 | NC_006548.1 | + | 31248 | 0.66 | 0.547847 |
Target: 5'- gGUCGGUgacCCAgCCgaGCGaCUC-UGCGUGGa -3' miRNA: 3'- -CAGCCGa--GGU-GG--UGC-GAGaACGUACC- -5' |
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30501 | 5' | -55.8 | NC_006548.1 | + | 11688 | 0.69 | 0.394035 |
Target: 5'- -cCGGCgCCGCCGCGCgccgcGCAUGa -3' miRNA: 3'- caGCCGaGGUGGUGCGagaa-CGUACc -5' |
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30501 | 5' | -55.8 | NC_006548.1 | + | 10106 | 0.73 | 0.219116 |
Target: 5'- -aCGGCUCCugaugcGCuCGCGCUUgcUGCGUGGg -3' miRNA: 3'- caGCCGAGG------UG-GUGCGAGa-ACGUACC- -5' |
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30501 | 5' | -55.8 | NC_006548.1 | + | 6582 | 1.09 | 0.000518 |
Target: 5'- uGUCGGCUCCACCACGCUCUUGCAUGGc -3' miRNA: 3'- -CAGCCGAGGUGGUGCGAGAACGUACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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