Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30502 | 3' | -56.7 | NC_006548.1 | + | 1259 | 0.66 | 0.575968 |
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Target: 5'- --cGCG-CGGUgCGGCAGGCUUgGCu- -3' miRNA: 3'- ccaCGCaGCCA-GCUGUCUGAGgCGug -5' |
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| 30502 | 3' | -56.7 | NC_006548.1 | + | 27002 | 0.66 | 0.563941 |
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Target: 5'- --aGCGUCGG-CGaguccgccgaucaGCAGACggcCCGCAUc -3' miRNA: 3'- ccaCGCAGCCaGC-------------UGUCUGa--GGCGUG- -5' |
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| 30502 | 3' | -56.7 | NC_006548.1 | + | 33103 | 0.66 | 0.554152 |
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Target: 5'- aGG-GUGUCGGUCaGACGGG-UCaGCGCc -3' miRNA: 3'- -CCaCGCAGCCAG-CUGUCUgAGgCGUG- -5' |
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| 30502 | 3' | -56.7 | NC_006548.1 | + | 33730 | 0.66 | 0.543334 |
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Target: 5'- --gGUGUCGGuuUCGugGGugaUCUGCACg -3' miRNA: 3'- ccaCGCAGCC--AGCugUCug-AGGCGUG- -5' |
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| 30502 | 3' | -56.7 | NC_006548.1 | + | 26734 | 0.7 | 0.348039 |
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Target: 5'- aGGUGaUGUCGGUCGACucgauguggaaagAGGCgaaGCACu -3' miRNA: 3'- -CCAC-GCAGCCAGCUG-------------UCUGaggCGUG- -5' |
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| 30502 | 3' | -56.7 | NC_006548.1 | + | 6978 | 1 | 0.002481 |
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Target: 5'- cGGUGCGUC-GUCGACAGACUCCGCACg -3' miRNA: 3'- -CCACGCAGcCAGCUGUCUGAGGCGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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