Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30502 | 5' | -55.7 | NC_006548.1 | + | 6357 | 0.66 | 0.605551 |
Target: 5'- gGGCGuUGAguucguccAGGUCgUUGACCGguUCGa -3' miRNA: 3'- aCCGC-GCU--------UCUAGgAGCUGGCguAGU- -5' |
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30502 | 5' | -55.7 | NC_006548.1 | + | 2623 | 0.66 | 0.583109 |
Target: 5'- gGGUGUGAcauGGA-CCagGACCGUAUCc -3' miRNA: 3'- aCCGCGCU---UCUaGGagCUGGCGUAGu -5' |
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30502 | 5' | -55.7 | NC_006548.1 | + | 4250 | 0.66 | 0.583109 |
Target: 5'- gGGCGCGGguGGcGUUgUUGACgGCAUCc -3' miRNA: 3'- aCCGCGCU--UC-UAGgAGCUGgCGUAGu -5' |
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30502 | 5' | -55.7 | NC_006548.1 | + | 18698 | 0.66 | 0.570834 |
Target: 5'- cUGGCcgagcuaGCGAcccugccgcAGAUCCUCGcccaGCCGCAg-- -3' miRNA: 3'- -ACCG-------CGCU---------UCUAGGAGC----UGGCGUagu -5' |
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30502 | 5' | -55.7 | NC_006548.1 | + | 9060 | 0.66 | 0.560837 |
Target: 5'- cGGCGCuccaGAAGccCCUCuGGCCGuCAUCc -3' miRNA: 3'- aCCGCG----CUUCuaGGAG-CUGGC-GUAGu -5' |
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30502 | 5' | -55.7 | NC_006548.1 | + | 16512 | 0.66 | 0.549788 |
Target: 5'- -aGCGCGuGGG-CUggGACCGCGUCAc -3' miRNA: 3'- acCGCGCuUCUaGGagCUGGCGUAGU- -5' |
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30502 | 5' | -55.7 | NC_006548.1 | + | 29324 | 0.66 | 0.547586 |
Target: 5'- gGGUauugucgagcugcugGCGGAGAUCCUucacgcgcucgaucgCGGCCGgAUCGc -3' miRNA: 3'- aCCG---------------CGCUUCUAGGA---------------GCUGGCgUAGU- -5' |
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30502 | 5' | -55.7 | NC_006548.1 | + | 19751 | 0.67 | 0.527901 |
Target: 5'- aGGCGuCGAuacGGGUUgUCGG-CGCGUCAc -3' miRNA: 3'- aCCGC-GCU---UCUAGgAGCUgGCGUAGU- -5' |
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30502 | 5' | -55.7 | NC_006548.1 | + | 22427 | 0.67 | 0.506346 |
Target: 5'- cGGUGCGguGAccaguUCCUUGACCGacUCAc -3' miRNA: 3'- aCCGCGCuuCU-----AGGAGCUGGCguAGU- -5' |
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30502 | 5' | -55.7 | NC_006548.1 | + | 32720 | 0.67 | 0.495709 |
Target: 5'- gGGCGCGAGcgcguGcUCCUCGACCuCAg-- -3' miRNA: 3'- aCCGCGCUU-----CuAGGAGCUGGcGUagu -5' |
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30502 | 5' | -55.7 | NC_006548.1 | + | 31957 | 0.69 | 0.424409 |
Target: 5'- aGGCGCGAGGAaguccaUCCggcacaGACCGaaAUCAg -3' miRNA: 3'- aCCGCGCUUCU------AGGag----CUGGCg-UAGU- -5' |
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30502 | 5' | -55.7 | NC_006548.1 | + | 33510 | 0.7 | 0.359598 |
Target: 5'- cGGCGgGcAGGUCUUCcaccGCCGCAUCc -3' miRNA: 3'- aCCGCgCuUCUAGGAGc---UGGCGUAGu -5' |
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30502 | 5' | -55.7 | NC_006548.1 | + | 7492 | 0.7 | 0.342394 |
Target: 5'- aGGCGCGAuGGucggcaccuugcUCCUCGGCCuGC-UCGg -3' miRNA: 3'- aCCGCGCUuCU------------AGGAGCUGG-CGuAGU- -5' |
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30502 | 5' | -55.7 | NC_006548.1 | + | 18157 | 0.72 | 0.272445 |
Target: 5'- aGGCcaggugcaguucGCGggGAUgUUCGACgGCAUCGc -3' miRNA: 3'- aCCG------------CGCuuCUAgGAGCUGgCGUAGU- -5' |
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30502 | 5' | -55.7 | NC_006548.1 | + | 7015 | 1.09 | 0.000592 |
Target: 5'- cUGGCGCGAAGAUCCUCGACCGCAUCAg -3' miRNA: 3'- -ACCGCGCUUCUAGGAGCUGGCGUAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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