Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30503 | 5' | -58 | NC_006548.1 | + | 12455 | 0.66 | 0.400545 |
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Target: 5'- -cGCUGGGCAuucguacugUCCGcuuacaacGGCGGCCAa-- -3' miRNA: 3'- cuUGACCCGU---------AGGUc-------CCGCCGGUaua -5' |
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| 30503 | 5' | -58 | NC_006548.1 | + | 21260 | 0.66 | 0.381912 |
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Target: 5'- -uGCUaccGGCAgCCuGGGCGGCCAg-- -3' miRNA: 3'- cuUGAc--CCGUaGGuCCCGCCGGUaua -5' |
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| 30503 | 5' | -58 | NC_006548.1 | + | 12408 | 0.67 | 0.363867 |
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Target: 5'- cGGCUGGuuGgugCCgAGGGCGGCCGg-- -3' miRNA: 3'- cUUGACCcgUa--GG-UCCCGCCGGUaua -5' |
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| 30503 | 5' | -58 | NC_006548.1 | + | 15421 | 0.67 | 0.355068 |
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Target: 5'- ----aGGGCAUCCugcAGGGUGaCCGUGUg -3' miRNA: 3'- cuugaCCCGUAGG---UCCCGCcGGUAUA- -5' |
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| 30503 | 5' | -58 | NC_006548.1 | + | 37960 | 0.68 | 0.321391 |
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Target: 5'- uGAGCUGGGUG-CCGGcGGUgagguaGGCCGUGc -3' miRNA: 3'- -CUUGACCCGUaGGUC-CCG------CCGGUAUa -5' |
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| 30503 | 5' | -58 | NC_006548.1 | + | 15594 | 0.68 | 0.282734 |
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Target: 5'- uAACUGGcaGCAgCCcgAGGGCGGCCGa-- -3' miRNA: 3'- cUUGACC--CGUaGG--UCCCGCCGGUaua -5' |
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| 30503 | 5' | -58 | NC_006548.1 | + | 659 | 0.69 | 0.247194 |
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Target: 5'- aGGGcCUGGGCAucgagcaUCCAGaacaGGCGGCCGg-- -3' miRNA: 3'- -CUU-GACCCGU-------AGGUC----CCGCCGGUaua -5' |
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| 30503 | 5' | -58 | NC_006548.1 | + | 7354 | 1.05 | 0.000481 |
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Target: 5'- cGAACUGGGCAUCCAGGGCGGCCAUAUc -3' miRNA: 3'- -CUUGACCCGUAGGUCCCGCCGGUAUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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