Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30504 | 5' | -53.5 | NC_006548.1 | + | 5424 | 0.66 | 0.709676 |
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Target: 5'- uGGGCCAUGgcgcgcaguuugauGAcGUGCGCCaggCAUUu -3' miRNA: 3'- -CCCGGUACau------------CUuCGCGCGGa--GUAAc -5' |
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| 30504 | 5' | -53.5 | NC_006548.1 | + | 8106 | 0.66 | 0.708552 |
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Target: 5'- -aGCCAUGcUGGAAGCcggaaaGCGCCUgAUc- -3' miRNA: 3'- ccCGGUAC-AUCUUCG------CGCGGAgUAac -5' |
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| 30504 | 5' | -53.5 | NC_006548.1 | + | 2416 | 0.67 | 0.640034 |
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Target: 5'- cGGCC-UGgcG-GGUGCGCCUCGc-- -3' miRNA: 3'- cCCGGuACauCuUCGCGCGGAGUaac -5' |
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| 30504 | 5' | -53.5 | NC_006548.1 | + | 20047 | 0.68 | 0.59403 |
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Target: 5'- uGGGCCAaggGUcuGGAGGUGCGCgaCGg-- -3' miRNA: 3'- -CCCGGUa--CA--UCUUCGCGCGgaGUaac -5' |
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| 30504 | 5' | -53.5 | NC_006548.1 | + | 27381 | 0.68 | 0.556469 |
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Target: 5'- cGGGCCGUGUugAGGccaaugccuaugccAGCGCGCUgaaCAg-- -3' miRNA: 3'- -CCCGGUACA--UCU--------------UCGCGCGGa--GUaac -5' |
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| 30504 | 5' | -53.5 | NC_006548.1 | + | 4857 | 0.68 | 0.548583 |
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Target: 5'- aGGCUcgGUGcaauugcgcGAAGCGUGCCUCc--- -3' miRNA: 3'- cCCGGuaCAU---------CUUCGCGCGGAGuaac -5' |
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| 30504 | 5' | -53.5 | NC_006548.1 | + | 27041 | 0.71 | 0.411055 |
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Target: 5'- aGGGCCAUGgu--GGCG-GCCUCGc-- -3' miRNA: 3'- -CCCGGUACaucuUCGCgCGGAGUaac -5' |
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| 30504 | 5' | -53.5 | NC_006548.1 | + | 8078 | 1.11 | 0.000612 |
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Target: 5'- uGGGCCAUGUAGAAGCGCGCCUCAUUGa -3' miRNA: 3'- -CCCGGUACAUCUUCGCGCGGAGUAAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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