miRNA: 3'- aCGACGA---CCGGCCGcCa---AAGAa----GAGC- -5'
miRNA: 3'- aCGACGaCCGGCCGccaAAGaaGAGC- -5'
miRNA: 3'- aCGACG--ACCGGCCGCcaAAGAAGagc -5'
miRNA: 3'- -ACGACGACCGGCCGCCAAAGAAGAGC- -5'
Results 1 - 4 of 4 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30507 | 3' | -56.6 | NC_006548.1 | + | 574 | 0.66 | 0.538806 |
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Target: 5'- aGCUGUUccaGGCCGGCcGccugUUCUggaugCUCGa -3' miRNA: 3'- aCGACGA---CCGGCCGcCa---AAGAa----GAGC- -5' |
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| 30507 | 3' | -56.6 | NC_006548.1 | + | 34131 | 0.66 | 0.538806 |
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Target: 5'- gGCUGUcGGCCGGCcagUUCacCUCGu -3' miRNA: 3'- aCGACGaCCGGCCGccaAAGaaGAGC- -5' |
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| 30507 | 3' | -56.6 | NC_006548.1 | + | 16794 | 0.68 | 0.424409 |
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Target: 5'- gGCUGUacUGGCCGGUG--UUCUUCa-- -3' miRNA: 3'- aCGACG--ACCGGCCGCcaAAGAAGagc -5' |
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| 30507 | 3' | -56.6 | NC_006548.1 | + | 12098 | 1.08 | 0.000478 |
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Target: 5'- cUGCUGCUGGCCGGCGGUUUCUUCUCGu -3' miRNA: 3'- -ACGACGACCGGCCGCCAAAGAAGAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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