Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30508 | 3' | -57.9 | NC_006548.1 | + | 4254 | 0.66 | 0.478284 |
Target: 5'- cGGUGcucGAUCGCCGGC-UGUucAUUCGuGCg -3' miRNA: 3'- cUCAC---CUAGCGGCCGgACA--UAGGC-CG- -5' |
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30508 | 3' | -57.9 | NC_006548.1 | + | 1468 | 0.66 | 0.468104 |
Target: 5'- cGAGgcGGAUCGCCGGCa-GUGgagUGGUa -3' miRNA: 3'- -CUCa-CCUAGCGGCCGgaCAUag-GCCG- -5' |
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30508 | 3' | -57.9 | NC_006548.1 | + | 31044 | 0.66 | 0.458038 |
Target: 5'- gGAGUGGAccaCGuuGuuuuccaaaccGCCUGgcgcUCCGGCa -3' miRNA: 3'- -CUCACCUa--GCggC-----------CGGACau--AGGCCG- -5' |
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30508 | 3' | -57.9 | NC_006548.1 | + | 37785 | 0.66 | 0.458038 |
Target: 5'- cGAGUaGA-CGCCGGUCg----CCGGCa -3' miRNA: 3'- -CUCAcCUaGCGGCCGGacauaGGCCG- -5' |
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30508 | 3' | -57.9 | NC_006548.1 | + | 11220 | 0.67 | 0.428555 |
Target: 5'- -uGUGGAUCGCCGaGCaa----CCGGUg -3' miRNA: 3'- cuCACCUAGCGGC-CGgacauaGGCCG- -5' |
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30508 | 3' | -57.9 | NC_006548.1 | + | 6386 | 0.67 | 0.425668 |
Target: 5'- aAGUGGcgCGCCcacuucaaugcgacGGCC-GUAcacagCCGGCa -3' miRNA: 3'- cUCACCuaGCGG--------------CCGGaCAUa----GGCCG- -5' |
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30508 | 3' | -57.9 | NC_006548.1 | + | 27984 | 0.67 | 0.418978 |
Target: 5'- cGAGUGGcUgGaaaagGGCCUGauUCCGGCg -3' miRNA: 3'- -CUCACCuAgCgg---CCGGACauAGGCCG- -5' |
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30508 | 3' | -57.9 | NC_006548.1 | + | 2431 | 0.67 | 0.409533 |
Target: 5'- cGGaUGGAaaacCGCCGGCCUGg--CgGGUg -3' miRNA: 3'- cUC-ACCUa---GCGGCCGGACauaGgCCG- -5' |
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30508 | 3' | -57.9 | NC_006548.1 | + | 15223 | 0.68 | 0.361759 |
Target: 5'- ---aGGAugcguccgccgcgcUUGCCGGCCUGcgcGUCCGGg -3' miRNA: 3'- cucaCCU--------------AGCGGCCGGACa--UAGGCCg -5' |
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30508 | 3' | -57.9 | NC_006548.1 | + | 19959 | 0.7 | 0.28479 |
Target: 5'- uGAG-GaGAUCGCCGcGaCCUacgAUCCGGCg -3' miRNA: 3'- -CUCaC-CUAGCGGC-C-GGAca-UAGGCCG- -5' |
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30508 | 3' | -57.9 | NC_006548.1 | + | 32656 | 0.7 | 0.270624 |
Target: 5'- cGAGcaacgGcGAUCGCUGcGCCUGUcaccCCGGCg -3' miRNA: 3'- -CUCa----C-CUAGCGGC-CGGACAua--GGCCG- -5' |
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30508 | 3' | -57.9 | NC_006548.1 | + | 12493 | 0.73 | 0.1727 |
Target: 5'- aGGGUGGGUaaaucgcgaccguaaGCUGGCCUcgGcAUCCGGCg -3' miRNA: 3'- -CUCACCUAg--------------CGGCCGGA--CaUAGGCCG- -5' |
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30508 | 3' | -57.9 | NC_006548.1 | + | 12322 | 1.1 | 0.000298 |
Target: 5'- uGAGUGGAUCGCCGGCCUGUAUCCGGCc -3' miRNA: 3'- -CUCACCUAGCGGCCGGACAUAGGCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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