Results 1 - 20 of 21 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30510 | 3' | -63.5 | NC_006548.1 | + | 5132 | 0.66 | 0.25541 |
Target: 5'- gGUGGGUGagucCGgCGCCGGUaaauccacccUGCGCCGcGa -3' miRNA: 3'- -CACCCAU----GCgGUGGCCG----------GCGCGGCuC- -5' |
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30510 | 3' | -63.5 | NC_006548.1 | + | 12495 | 0.66 | 0.24291 |
Target: 5'- gGUGGGUaaaucGCGaCCguaaGCUGGCCucggcauccgGCGCCGAc -3' miRNA: 3'- -CACCCA-----UGC-GG----UGGCCGG----------CGCGGCUc -5' |
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30510 | 3' | -63.5 | NC_006548.1 | + | 6294 | 0.67 | 0.21944 |
Target: 5'- --uGGUGCGCCuauGCCGGCUGUGUaCGGc -3' miRNA: 3'- cacCCAUGCGG---UGGCCGGCGCG-GCUc -5' |
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30510 | 3' | -63.5 | NC_006548.1 | + | 13278 | 0.67 | 0.213883 |
Target: 5'- cUGGGUGCGCgacuucaaCACCGcCCucgGCGCCGGu -3' miRNA: 3'- cACCCAUGCG--------GUGGCcGG---CGCGGCUc -5' |
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30510 | 3' | -63.5 | NC_006548.1 | + | 28252 | 0.67 | 0.213883 |
Target: 5'- cUGGGUACuGCCAUCGGUuaUGCgGCCa-- -3' miRNA: 3'- cACCCAUG-CGGUGGCCG--GCG-CGGcuc -5' |
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30510 | 3' | -63.5 | NC_006548.1 | + | 21446 | 0.67 | 0.213883 |
Target: 5'- -cGGGUccgucuacauCGCC-CCGGCCGUucuGCCGGu -3' miRNA: 3'- caCCCAu---------GCGGuGGCCGGCG---CGGCUc -5' |
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30510 | 3' | -63.5 | NC_006548.1 | + | 17256 | 0.67 | 0.208447 |
Target: 5'- --cGGUcaGCCaACgGGCCGgGCCGAGg -3' miRNA: 3'- cacCCAugCGG-UGgCCGGCgCGGCUC- -5' |
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30510 | 3' | -63.5 | NC_006548.1 | + | 13365 | 0.67 | 0.203131 |
Target: 5'- cUGGcc-CGCCGCCGGUucUGaCGCCGAGc -3' miRNA: 3'- cACCcauGCGGUGGCCG--GC-GCGGCUC- -5' |
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30510 | 3' | -63.5 | NC_006548.1 | + | 11754 | 0.68 | 0.178288 |
Target: 5'- cUGGucaUGCGCggCGCgCGGCgGCGCCGGGg -3' miRNA: 3'- cACCc--AUGCG--GUG-GCCGgCGCGGCUC- -5' |
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30510 | 3' | -63.5 | NC_006548.1 | + | 23776 | 0.68 | 0.169128 |
Target: 5'- uUGGGUGaucgUGCCAaguUCGGCgGCGCCGc- -3' miRNA: 3'- cACCCAU----GCGGU---GGCCGgCGCGGCuc -5' |
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30510 | 3' | -63.5 | NC_006548.1 | + | 32893 | 0.69 | 0.149646 |
Target: 5'- -cGGGUAuCGCacucgaccguuugugCGCCGGCCGCGgUGAc -3' miRNA: 3'- caCCCAU-GCG---------------GUGGCCGGCGCgGCUc -5' |
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30510 | 3' | -63.5 | NC_006548.1 | + | 33945 | 0.69 | 0.139182 |
Target: 5'- --cGGUcauGCGCCguccaucgucgcucACCGGCCGCGCgCGAa -3' miRNA: 3'- cacCCA---UGCGG--------------UGGCCGGCGCG-GCUc -5' |
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30510 | 3' | -63.5 | NC_006548.1 | + | 21125 | 0.7 | 0.119266 |
Target: 5'- uGUGGGUAacgGCC-UCGGUCGUGCUGAu -3' miRNA: 3'- -CACCCAUg--CGGuGGCCGGCGCGGCUc -5' |
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30510 | 3' | -63.5 | NC_006548.1 | + | 1185 | 0.71 | 0.10119 |
Target: 5'- -cGGGUucACGCCaggaGCCGGCUGCaaaGCCGGu -3' miRNA: 3'- caCCCA--UGCGG----UGGCCGGCG---CGGCUc -5' |
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30510 | 3' | -63.5 | NC_006548.1 | + | 17135 | 0.71 | 0.10119 |
Target: 5'- cUGGGUAcuCGCCACCuGGCCGaGcCCGAc -3' miRNA: 3'- cACCCAU--GCGGUGG-CCGGCgC-GGCUc -5' |
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30510 | 3' | -63.5 | NC_006548.1 | + | 33047 | 0.72 | 0.095761 |
Target: 5'- -gGGGUACaagcgggaGCCgGCCGGCCaGCGCCGc- -3' miRNA: 3'- caCCCAUG--------CGG-UGGCCGG-CGCGGCuc -5' |
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30510 | 3' | -63.5 | NC_006548.1 | + | 2007 | 0.72 | 0.090608 |
Target: 5'- -gGGGUAUGCCGCgGGCaagCGCGCgCGGc -3' miRNA: 3'- caCCCAUGCGGUGgCCG---GCGCG-GCUc -5' |
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30510 | 3' | -63.5 | NC_006548.1 | + | 23868 | 0.73 | 0.074574 |
Target: 5'- -cGGGUcGCGCCACCGGCaUG-GCCGGc -3' miRNA: 3'- caCCCA-UGCGGUGGCCG-GCgCGGCUc -5' |
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30510 | 3' | -63.5 | NC_006548.1 | + | 35943 | 0.76 | 0.047523 |
Target: 5'- -----cACGCCGCCGGCCGCGCgGAu -3' miRNA: 3'- cacccaUGCGGUGGCCGGCGCGgCUc -5' |
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30510 | 3' | -63.5 | NC_006548.1 | + | 28607 | 0.78 | 0.031898 |
Target: 5'- -cGGGUaGCGCCGCgaccUGGCCgGCGCCGAGc -3' miRNA: 3'- caCCCA-UGCGGUG----GCCGG-CGCGGCUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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