Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30511 | 3' | -54.6 | NC_006548.1 | + | 13020 | 0.66 | 0.61771 |
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Target: 5'- -aGCCGuGgauCACCA-CGGCUGCGc- -3' miRNA: 3'- agCGGCuCau-GUGGUaGCUGACGCuu -5' |
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| 30511 | 3' | -54.6 | NC_006548.1 | + | 28249 | 0.67 | 0.583588 |
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Target: 5'- -gGCCuGGGUACuGCCAUCGGuuaUGCGGc -3' miRNA: 3'- agCGG-CUCAUG-UGGUAGCUg--ACGCUu -5' |
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| 30511 | 3' | -54.6 | NC_006548.1 | + | 23872 | 0.67 | 0.572289 |
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Target: 5'- cUGCCGGGUcGCGCCAcCGGCauggccgGCGGg -3' miRNA: 3'- aGCGGCUCA-UGUGGUaGCUGa------CGCUu -5' |
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| 30511 | 3' | -54.6 | NC_006548.1 | + | 22627 | 0.67 | 0.561042 |
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Target: 5'- aCGCCGgccAGUugGCCGUCGAUcagGCu-- -3' miRNA: 3'- aGCGGC---UCAugUGGUAGCUGa--CGcuu -5' |
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| 30511 | 3' | -54.6 | NC_006548.1 | + | 29361 | 0.68 | 0.51675 |
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Target: 5'- cUCGCCGAugGCACCcuGUCG-CUGCaGGAg -3' miRNA: 3'- -AGCGGCUcaUGUGG--UAGCuGACG-CUU- -5' |
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| 30511 | 3' | -54.6 | NC_006548.1 | + | 2160 | 0.68 | 0.51675 |
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Target: 5'- -gGCCGuGUACGCCAaggUCGACcaguucGCGGg -3' miRNA: 3'- agCGGCuCAUGUGGU---AGCUGa-----CGCUu -5' |
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| 30511 | 3' | -54.6 | NC_006548.1 | + | 20278 | 0.69 | 0.443008 |
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Target: 5'- uUCGCCGAGgaugACggcgcccugGCCAUCGAgUucGCGGAa -3' miRNA: 3'- -AGCGGCUCa---UG---------UGGUAGCUgA--CGCUU- -5' |
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| 30511 | 3' | -54.6 | NC_006548.1 | + | 21321 | 0.7 | 0.375574 |
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Target: 5'- aCGUCGAagaccGCACCGUCGGCgGCGGGa -3' miRNA: 3'- aGCGGCUca---UGUGGUAGCUGaCGCUU- -5' |
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| 30511 | 3' | -54.6 | NC_006548.1 | + | 14137 | 1.06 | 0.001073 |
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Target: 5'- gUCGCCGAGUACACCAUCGACUGCGAAc -3' miRNA: 3'- -AGCGGCUCAUGUGGUAGCUGACGCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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