Results 1 - 20 of 34 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30512 | 5' | -58.1 | NC_006548.1 | + | 4653 | 0.66 | 0.44582 |
Target: 5'- uUGCuGCCGUGgAaaagcaccuggaguUcCGCUuCGGCCGGGc -3' miRNA: 3'- -ACGuCGGCACgU--------------A-GCGAuGCCGGUCU- -5' |
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30512 | 5' | -58.1 | NC_006548.1 | + | 22588 | 0.66 | 0.43887 |
Target: 5'- gGCGGCUGUGCGccgguaguggUUGgUGCGG-CAGGu -3' miRNA: 3'- aCGUCGGCACGU----------AGCgAUGCCgGUCU- -5' |
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30512 | 5' | -58.1 | NC_006548.1 | + | 1087 | 0.66 | 0.43887 |
Target: 5'- cGCAGCUcaGCAUCGCUgACGcCCAa- -3' miRNA: 3'- aCGUCGGcaCGUAGCGA-UGCcGGUcu -5' |
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30512 | 5' | -58.1 | NC_006548.1 | + | 29281 | 0.66 | 0.429048 |
Target: 5'- cGCGGCCGgaucGCcaGUUGCcuUGGCCAGc -3' miRNA: 3'- aCGUCGGCa---CG--UAGCGauGCCGGUCu -5' |
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30512 | 5' | -58.1 | NC_006548.1 | + | 22303 | 0.66 | 0.429048 |
Target: 5'- gUGguGCCGuUGCGggUGU--CGGCCAGGc -3' miRNA: 3'- -ACguCGGC-ACGUa-GCGauGCCGGUCU- -5' |
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30512 | 5' | -58.1 | NC_006548.1 | + | 36439 | 0.66 | 0.409801 |
Target: 5'- cGCGGCUGaGCAgCGCguaguucaucUGCaGGCCGGAc -3' miRNA: 3'- aCGUCGGCaCGUaGCG----------AUG-CCGGUCU- -5' |
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30512 | 5' | -58.1 | NC_006548.1 | + | 33962 | 0.66 | 0.400382 |
Target: 5'- cGCAGCUGUaccGUggCGCU-CGGCCGu- -3' miRNA: 3'- aCGUCGGCA---CGuaGCGAuGCCGGUcu -5' |
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30512 | 5' | -58.1 | NC_006548.1 | + | 36363 | 0.66 | 0.400381 |
Target: 5'- cUGCAGCUGcgccagaucUGCAUCGCUcaguGCcGCCGcGAg -3' miRNA: 3'- -ACGUCGGC---------ACGUAGCGA----UGcCGGU-CU- -5' |
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30512 | 5' | -58.1 | NC_006548.1 | + | 18459 | 0.66 | 0.400381 |
Target: 5'- cGCAGCCGguucGgGUgGCaGCGGCguGGu -3' miRNA: 3'- aCGUCGGCa---CgUAgCGaUGCCGguCU- -5' |
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30512 | 5' | -58.1 | NC_006548.1 | + | 7608 | 0.67 | 0.391101 |
Target: 5'- cGCAGCUcUGCcgCGCgcugguCGGCCGu- -3' miRNA: 3'- aCGUCGGcACGuaGCGau----GCCGGUcu -5' |
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30512 | 5' | -58.1 | NC_006548.1 | + | 18221 | 0.67 | 0.391101 |
Target: 5'- --gAGCUG-GCAUCGCUgggcaaggACGGcCCGGAc -3' miRNA: 3'- acgUCGGCaCGUAGCGA--------UGCC-GGUCU- -5' |
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30512 | 5' | -58.1 | NC_006548.1 | + | 28530 | 0.67 | 0.391101 |
Target: 5'- cGCAGUCcUGC-UCgGCgcCGGCCAGGu -3' miRNA: 3'- aCGUCGGcACGuAG-CGauGCCGGUCU- -5' |
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30512 | 5' | -58.1 | NC_006548.1 | + | 35084 | 0.67 | 0.381962 |
Target: 5'- aGCgaaAGCCGacaguuUGCGcuUUGCUACGGCCGc- -3' miRNA: 3'- aCG---UCGGC------ACGU--AGCGAUGCCGGUcu -5' |
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30512 | 5' | -58.1 | NC_006548.1 | + | 33756 | 0.67 | 0.372966 |
Target: 5'- aGCGGUCG---GUCGCcgAUGGCCAGGc -3' miRNA: 3'- aCGUCGGCacgUAGCGa-UGCCGGUCU- -5' |
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30512 | 5' | -58.1 | NC_006548.1 | + | 1937 | 0.67 | 0.364115 |
Target: 5'- gUGCAGCCGcGCG-CGCUugcccGCGGCa--- -3' miRNA: 3'- -ACGUCGGCaCGUaGCGA-----UGCCGgucu -5' |
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30512 | 5' | -58.1 | NC_006548.1 | + | 21270 | 0.67 | 0.364115 |
Target: 5'- aGCAGCgcaGUGCuaccggcaGCcuggGCGGCCAGGc -3' miRNA: 3'- aCGUCGg--CACGuag-----CGa---UGCCGGUCU- -5' |
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30512 | 5' | -58.1 | NC_006548.1 | + | 26340 | 0.67 | 0.355411 |
Target: 5'- gGCGGCCGg--GUCG--AUGGCCAGAg -3' miRNA: 3'- aCGUCGGCacgUAGCgaUGCCGGUCU- -5' |
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30512 | 5' | -58.1 | NC_006548.1 | + | 33364 | 0.67 | 0.355411 |
Target: 5'- cGCAGCgccGCAU-GCUccucGCGGCCGGAg -3' miRNA: 3'- aCGUCGgcaCGUAgCGA----UGCCGGUCU- -5' |
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30512 | 5' | -58.1 | NC_006548.1 | + | 36955 | 0.67 | 0.346854 |
Target: 5'- aGCAGCCGguUGCGgugCGCccacgacACGGUCAGc -3' miRNA: 3'- aCGUCGGC--ACGUa--GCGa------UGCCGGUCu -5' |
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30512 | 5' | -58.1 | NC_006548.1 | + | 16042 | 0.68 | 0.327738 |
Target: 5'- cGCAGCCGU-CGucugcuuggugaccUCGCgcaagGCGGCgCGGAu -3' miRNA: 3'- aCGUCGGCAcGU--------------AGCGa----UGCCG-GUCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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