Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30513 | 5' | -58.4 | NC_006548.1 | + | 28088 | 0.66 | 0.421872 |
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Target: 5'- cUGCAGGGccgcgccgaaCCgCGGCAGAaacUCGGCCg -3' miRNA: 3'- uACGUCUUca--------GG-GCCGUCU---AGCCGGa -5' |
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| 30513 | 5' | -58.4 | NC_006548.1 | + | 14626 | 0.66 | 0.384227 |
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Target: 5'- -cGCugaAGAAGUUCCaGGCAGA--GGCCg -3' miRNA: 3'- uaCG---UCUUCAGGG-CCGUCUagCCGGa -5' |
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| 30513 | 5' | -58.4 | NC_006548.1 | + | 29897 | 0.67 | 0.366259 |
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Target: 5'- -aGCAGcGGggucaugCCCGGCuGGguugCGGCCg -3' miRNA: 3'- uaCGUCuUCa------GGGCCGuCUa---GCCGGa -5' |
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| 30513 | 5' | -58.4 | NC_006548.1 | + | 36986 | 0.67 | 0.357494 |
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Target: 5'- -cGCAGAcgAGUCaaCCaGUuGAUCGGCCUg -3' miRNA: 3'- uaCGUCU--UCAG--GGcCGuCUAGCCGGA- -5' |
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| 30513 | 5' | -58.4 | NC_006548.1 | + | 31467 | 0.67 | 0.34041 |
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Target: 5'- -gGCAGGucAGUUCCGGCcggugauGAUUGGCUc -3' miRNA: 3'- uaCGUCU--UCAGGGCCGu------CUAGCCGGa -5' |
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| 30513 | 5' | -58.4 | NC_006548.1 | + | 36238 | 0.69 | 0.278079 |
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Target: 5'- cUGCGGAcGUCCCGGCugc-CGGCa- -3' miRNA: 3'- uACGUCUuCAGGGCCGucuaGCCGga -5' |
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| 30513 | 5' | -58.4 | NC_006548.1 | + | 14875 | 1.06 | 0.000415 |
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Target: 5'- cAUGCAGAAGUCCCGGCAGAUCGGCCUg -3' miRNA: 3'- -UACGUCUUCAGGGCCGUCUAGCCGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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