Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30514 | 3' | -50 | NC_006548.1 | + | 16112 | 0.66 | 0.881943 |
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Target: 5'- --cGAcUAUCAGuCCgccgGCACACGGacgCAg -3' miRNA: 3'- cuuCUuAUAGUC-GGa---CGUGUGCCa--GU- -5' |
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| 30514 | 3' | -50 | NC_006548.1 | + | 25858 | 0.66 | 0.856197 |
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Target: 5'- --cGGAUGUCAccGCCUGCuggcccGCGCGGg-- -3' miRNA: 3'- cuuCUUAUAGU--CGGACG------UGUGCCagu -5' |
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| 30514 | 3' | -50 | NC_006548.1 | + | 33129 | 0.66 | 0.846127 |
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Target: 5'- cGAGGAAccgC-GCCUGCGCGCuguagagggugucGGUCAg -3' miRNA: 3'- -CUUCUUauaGuCGGACGUGUG-------------CCAGU- -5' |
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| 30514 | 3' | -50 | NC_006548.1 | + | 35519 | 0.67 | 0.818102 |
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Target: 5'- cAAGGAUcgCGGCCUGCugGaccaGGcgUCAu -3' miRNA: 3'- cUUCUUAuaGUCGGACGugUg---CC--AGU- -5' |
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| 30514 | 3' | -50 | NC_006548.1 | + | 6123 | 0.67 | 0.80798 |
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Target: 5'- cGAAGAug--CAGUgcaaCUGguCACGGUCAa -3' miRNA: 3'- -CUUCUuauaGUCG----GACguGUGCCAGU- -5' |
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| 30514 | 3' | -50 | NC_006548.1 | + | 22291 | 0.68 | 0.776376 |
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Target: 5'- --cGggUGUCGGCCagGCGauCGCGGUa- -3' miRNA: 3'- cuuCuuAUAGUCGGa-CGU--GUGCCAgu -5' |
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| 30514 | 3' | -50 | NC_006548.1 | + | 23252 | 0.68 | 0.765475 |
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Target: 5'- aGAAGAGaucgaaGUCGGCCaUGC-CACGGUgGa -3' miRNA: 3'- -CUUCUUa-----UAGUCGG-ACGuGUGCCAgU- -5' |
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| 30514 | 3' | -50 | NC_006548.1 | + | 28652 | 0.72 | 0.555555 |
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Target: 5'- uGAAGggUGUCuuggugguggGGCCUGUAC-CGGUg- -3' miRNA: 3'- -CUUCuuAUAG----------UCGGACGUGuGCCAgu -5' |
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| 30514 | 3' | -50 | NC_006548.1 | + | 29386 | 0.72 | 0.521 |
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Target: 5'- aGGAGGccgcgGUAUCGuuCCUGCAgGCGGUCAg -3' miRNA: 3'- -CUUCU-----UAUAGUc-GGACGUgUGCCAGU- -5' |
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| 30514 | 3' | -50 | NC_006548.1 | + | 15341 | 1.09 | 0.001811 |
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Target: 5'- cGAAGAAUAUCAGCCUGCACACGGUCAc -3' miRNA: 3'- -CUUCUUAUAGUCGGACGUGUGCCAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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