Results 1 - 20 of 53 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30515 | 3' | -55.9 | NC_006548.1 | + | 16720 | 0.66 | 0.561042 |
Target: 5'- -aGCaggCGCAGGCCGCcgucCUGGUcauAGCg -3' miRNA: 3'- uaUGg--GCGUCUGGCGauu-GACCG---UCG- -5' |
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30515 | 3' | -55.9 | NC_006548.1 | + | 36250 | 0.66 | 0.549856 |
Target: 5'- -gACCCGCAGgcucaucgaggcGCCGUaccgUGAUcucgcGGCGGCa -3' miRNA: 3'- uaUGGGCGUC------------UGGCG----AUUGa----CCGUCG- -5' |
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30515 | 3' | -55.9 | NC_006548.1 | + | 24823 | 0.66 | 0.549856 |
Target: 5'- ---aCCGcCGGAgCGCUAcauccuGCUGGaCGGCa -3' miRNA: 3'- uaugGGC-GUCUgGCGAU------UGACC-GUCG- -5' |
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30515 | 3' | -55.9 | NC_006548.1 | + | 24734 | 0.66 | 0.549856 |
Target: 5'- cGUACCCaagacCAGuuCCGCgGGCgUGGUAGCa -3' miRNA: 3'- -UAUGGGc----GUCu-GGCGaUUG-ACCGUCG- -5' |
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30515 | 3' | -55.9 | NC_006548.1 | + | 21161 | 0.66 | 0.53874 |
Target: 5'- -cGCCCGCcGuAUCGUgauccaUGGCAGCu -3' miRNA: 3'- uaUGGGCGuC-UGGCGauug--ACCGUCG- -5' |
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30515 | 3' | -55.9 | NC_006548.1 | + | 6775 | 0.66 | 0.509137 |
Target: 5'- -cACCUGUAGGCCGCUAGCcuucuugUucgccccaggcuucaGGUAGUa -3' miRNA: 3'- uaUGGGCGUCUGGCGAUUG-------A---------------CCGUCG- -5' |
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30515 | 3' | -55.9 | NC_006548.1 | + | 29280 | 0.66 | 0.505889 |
Target: 5'- aAUACCCgGCuGGCCGCcGACcagcucacuuUGGCucuGCg -3' miRNA: 3'- -UAUGGG-CGuCUGGCGaUUG----------ACCGu--CG- -5' |
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30515 | 3' | -55.9 | NC_006548.1 | + | 36282 | 0.66 | 0.505889 |
Target: 5'- -aACCCGCGGGgUGCgguCgGGCGGg -3' miRNA: 3'- uaUGGGCGUCUgGCGauuGaCCGUCg -5' |
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30515 | 3' | -55.9 | NC_006548.1 | + | 5970 | 0.67 | 0.495128 |
Target: 5'- -gGCCCGCAaaGCUGUUGaccaACUGGCcuauGGCg -3' miRNA: 3'- uaUGGGCGUc-UGGCGAU----UGACCG----UCG- -5' |
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30515 | 3' | -55.9 | NC_006548.1 | + | 19868 | 0.67 | 0.495128 |
Target: 5'- --uUCgGCGGACCGaaAAUUGGUGGCu -3' miRNA: 3'- uauGGgCGUCUGGCgaUUGACCGUCG- -5' |
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30515 | 3' | -55.9 | NC_006548.1 | + | 35809 | 0.67 | 0.495128 |
Target: 5'- --uCCCgaGCAGcCCGCUGuccucaucgaauACgGGCAGCg -3' miRNA: 3'- uauGGG--CGUCuGGCGAU------------UGaCCGUCG- -5' |
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30515 | 3' | -55.9 | NC_006548.1 | + | 29185 | 0.67 | 0.485532 |
Target: 5'- cUGCCgCGCGGccaguUCGCUGACgagaccggccugcugGGCGGCc -3' miRNA: 3'- uAUGG-GCGUCu----GGCGAUUGa--------------CCGUCG- -5' |
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30515 | 3' | -55.9 | NC_006548.1 | + | 32142 | 0.67 | 0.473926 |
Target: 5'- gAUGCCgGCAacGACCGCUucGACcGGCcgaGGUa -3' miRNA: 3'- -UAUGGgCGU--CUGGCGA--UUGaCCG---UCG- -5' |
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30515 | 3' | -55.9 | NC_006548.1 | + | 35237 | 0.67 | 0.473926 |
Target: 5'- -cGCCC-CGGACCaaccgGUgaauccucGACUGGCGGCg -3' miRNA: 3'- uaUGGGcGUCUGG-----CGa-------UUGACCGUCG- -5' |
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30515 | 3' | -55.9 | NC_006548.1 | + | 20897 | 0.67 | 0.473926 |
Target: 5'- -aGCCgGCGG-CCGagguGCUGcGCAGCu -3' miRNA: 3'- uaUGGgCGUCuGGCgau-UGAC-CGUCG- -5' |
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30515 | 3' | -55.9 | NC_006548.1 | + | 29511 | 0.67 | 0.463497 |
Target: 5'- --cCCCGCAGuugccuucuggGCCaGCU-GCUGcGCGGCa -3' miRNA: 3'- uauGGGCGUC-----------UGG-CGAuUGAC-CGUCG- -5' |
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30515 | 3' | -55.9 | NC_006548.1 | + | 4804 | 0.67 | 0.463497 |
Target: 5'- -cGCCUGCgugaguuggaGGACgGCUucaccaAGCUGGuCAGCa -3' miRNA: 3'- uaUGGGCG----------UCUGgCGA------UUGACC-GUCG- -5' |
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30515 | 3' | -55.9 | NC_006548.1 | + | 4636 | 0.67 | 0.452166 |
Target: 5'- -cACCUGgAGuuCCGCUucggccgGGCUGGCAaGCa -3' miRNA: 3'- uaUGGGCgUCu-GGCGA-------UUGACCGU-CG- -5' |
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30515 | 3' | -55.9 | NC_006548.1 | + | 6454 | 0.67 | 0.443008 |
Target: 5'- -aACCCGC--GCCGCgc-CUGGC-GCg -3' miRNA: 3'- uaUGGGCGucUGGCGauuGACCGuCG- -5' |
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30515 | 3' | -55.9 | NC_006548.1 | + | 12933 | 0.67 | 0.443008 |
Target: 5'- -gGCUCGCGcuGAgCGCgguGCUGuGCGGCg -3' miRNA: 3'- uaUGGGCGU--CUgGCGau-UGAC-CGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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