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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30517 | 3' | -48.3 | NC_006548.1 | + | 33793 | 0.66 | 0.947814 |
Target: 5'- cGACuUCcgcCUCGGCCCg--AUCcgCUGGa -3' miRNA: 3'- -CUGuAGu--GAGCUGGGaaaUAGa-GACC- -5' |
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30517 | 3' | -48.3 | NC_006548.1 | + | 14166 | 0.66 | 0.944787 |
Target: 5'- cGGCAUCGCUCGcCCCaagaaguaCUGGu -3' miRNA: 3'- -CUGUAGUGAGCuGGGaaauaga-GACC- -5' |
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30517 | 3' | -48.3 | NC_006548.1 | + | 15795 | 0.67 | 0.912425 |
Target: 5'- cGACGcCAC-CGGCCUgggUAUCggCUGGg -3' miRNA: 3'- -CUGUaGUGaGCUGGGaa-AUAGa-GACC- -5' |
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30517 | 3' | -48.3 | NC_006548.1 | + | 35789 | 0.68 | 0.898095 |
Target: 5'- cACAUCGCUCGACggcacggcgaaCCaguucgUUGUCgUCUGGc -3' miRNA: 3'- cUGUAGUGAGCUG-----------GGa-----AAUAG-AGACC- -5' |
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30517 | 3' | -48.3 | NC_006548.1 | + | 16282 | 1.11 | 0.002596 |
Target: 5'- cGACAUCACUCGACCCUUUAUCUCUGGc -3' miRNA: 3'- -CUGUAGUGAGCUGGGAAAUAGAGACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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