Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30518 | 5' | -52.4 | NC_006548.1 | + | 36294 | 0.66 | 0.802869 |
Target: 5'- gCGgGUCGCaauaacccGCGGGGUGCgg-UCGGg -3' miRNA: 3'- -GCgCAGUGgu------CGUUCUACGacaAGCC- -5' |
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30518 | 5' | -52.4 | NC_006548.1 | + | 18770 | 0.66 | 0.802869 |
Target: 5'- gGCGaggaUCugCGGCAGGGUcGCUaGcUCGGc -3' miRNA: 3'- gCGC----AGugGUCGUUCUA-CGA-CaAGCC- -5' |
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30518 | 5' | -52.4 | NC_006548.1 | + | 33446 | 0.66 | 0.771038 |
Target: 5'- uGUGUCgACCAGCAccaggucGGcgGCaUGggUCGGa -3' miRNA: 3'- gCGCAG-UGGUCGU-------UCuaCG-ACa-AGCC- -5' |
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30518 | 5' | -52.4 | NC_006548.1 | + | 10286 | 0.66 | 0.761501 |
Target: 5'- gCGCGguagaccuCCAGCAAGA-GCUGcUCGu -3' miRNA: 3'- -GCGCagu-----GGUCGUUCUaCGACaAGCc -5' |
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30518 | 5' | -52.4 | NC_006548.1 | + | 12915 | 0.66 | 0.755076 |
Target: 5'- uGCG-CACCAGCAcguucuGGcucgcgcugagcgcgGUGCUGUgCGGc -3' miRNA: 3'- gCGCaGUGGUCGU------UC---------------UACGACAaGCC- -5' |
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30518 | 5' | -52.4 | NC_006548.1 | + | 9507 | 0.67 | 0.706646 |
Target: 5'- uCG-GUcCACCAGCAGGAgGCUGcUCuGGu -3' miRNA: 3'- -GCgCA-GUGGUCGUUCUaCGACaAG-CC- -5' |
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30518 | 5' | -52.4 | NC_006548.1 | + | 25651 | 0.68 | 0.672678 |
Target: 5'- uCGCGUUucuucaACUGGCGAc--GCUGUUCGGc -3' miRNA: 3'- -GCGCAG------UGGUCGUUcuaCGACAAGCC- -5' |
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30518 | 5' | -52.4 | NC_006548.1 | + | 13427 | 0.69 | 0.625711 |
Target: 5'- gGCGUCagaACCGGCGgcgggccAGGUcGCUGUUUGa -3' miRNA: 3'- gCGCAG---UGGUCGU-------UCUA-CGACAAGCc -5' |
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30518 | 5' | -52.4 | NC_006548.1 | + | 37778 | 0.69 | 0.615388 |
Target: 5'- aCGCcgGUCGCCGGCAcuguGAUGagcgUGUUCGa -3' miRNA: 3'- -GCG--CAGUGGUCGUu---CUACg---ACAAGCc -5' |
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30518 | 5' | -52.4 | NC_006548.1 | + | 37856 | 0.69 | 0.592505 |
Target: 5'- gGCaUCGCCAGCcgcagauugcAGAUGCcaggGUUCGGu -3' miRNA: 3'- gCGcAGUGGUCGu---------UCUACGa---CAAGCC- -5' |
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30518 | 5' | -52.4 | NC_006548.1 | + | 24744 | 0.7 | 0.525022 |
Target: 5'- -aCGUCACCGGCAAGAcccaGCcGUUCa- -3' miRNA: 3'- gcGCAGUGGUCGUUCUa---CGaCAAGcc -5' |
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30518 | 5' | -52.4 | NC_006548.1 | + | 19331 | 0.73 | 0.400712 |
Target: 5'- uGCGcUACCAGCuGGAcggcagcgagcUGCUGUUUGGu -3' miRNA: 3'- gCGCaGUGGUCGuUCU-----------ACGACAAGCC- -5' |
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30518 | 5' | -52.4 | NC_006548.1 | + | 20588 | 0.73 | 0.372662 |
Target: 5'- cCGCGUCAgCGGC-AGAgGCUGacCGGa -3' miRNA: 3'- -GCGCAGUgGUCGuUCUaCGACaaGCC- -5' |
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30518 | 5' | -52.4 | NC_006548.1 | + | 22007 | 0.74 | 0.32069 |
Target: 5'- gGUGaUCACCGGCAgcGGGUuGCUGgUCGGa -3' miRNA: 3'- gCGC-AGUGGUCGU--UCUA-CGACaAGCC- -5' |
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30518 | 5' | -52.4 | NC_006548.1 | + | 13726 | 0.76 | 0.267157 |
Target: 5'- gGCGUCcaGCCGGUAGGAgaGCUGUUCa- -3' miRNA: 3'- gCGCAG--UGGUCGUUCUa-CGACAAGcc -5' |
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30518 | 5' | -52.4 | NC_006548.1 | + | 16528 | 1.11 | 0.00103 |
Target: 5'- cCGCGUCACCAGCAAGAUGCUGUUCGGc -3' miRNA: 3'- -GCGCAGUGGUCGUUCUACGACAAGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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