Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30520 | 3' | -55.3 | NC_006548.1 | + | 19288 | 0.66 | 0.585557 |
Target: 5'- gGCuGCCaagGCGUGCCGU-CAGGCg-- -3' miRNA: 3'- -CGcCGGga-CGUACGGUAuGUCUGacu -5' |
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30520 | 3' | -55.3 | NC_006548.1 | + | 33339 | 0.66 | 0.57313 |
Target: 5'- --cGCCCUGCGUGgCcgGCAGAaagcgauCUGGa -3' miRNA: 3'- cgcCGGGACGUACgGuaUGUCU-------GACU- -5' |
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30520 | 3' | -55.3 | NC_006548.1 | + | 2789 | 0.66 | 0.563009 |
Target: 5'- aGCGugcuuGCCCUGCGccGCC-UGCAGAUcGAu -3' miRNA: 3'- -CGC-----CGGGACGUa-CGGuAUGUCUGaCU- -5' |
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30520 | 3' | -55.3 | NC_006548.1 | + | 27029 | 0.67 | 0.540699 |
Target: 5'- aCGGCCC-GCAUcaggGCCAUGguGGCg-- -3' miRNA: 3'- cGCCGGGaCGUA----CGGUAUguCUGacu -5' |
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30520 | 3' | -55.3 | NC_006548.1 | + | 32437 | 0.68 | 0.475827 |
Target: 5'- cGCGGCgCUGCAggaGCgCAUcUGGACUGu -3' miRNA: 3'- -CGCCGgGACGUa--CG-GUAuGUCUGACu -5' |
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30520 | 3' | -55.3 | NC_006548.1 | + | 7414 | 0.68 | 0.475827 |
Target: 5'- gGCcGCCCUGgAUGCCcaguucGCGGACgGAg -3' miRNA: 3'- -CGcCGGGACgUACGGua----UGUCUGaCU- -5' |
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30520 | 3' | -55.3 | NC_006548.1 | + | 25928 | 0.68 | 0.465384 |
Target: 5'- aCGGCCCgcGCggGCCA-GCAGGCggUGAc -3' miRNA: 3'- cGCCGGGa-CGuaCGGUaUGUCUG--ACU- -5' |
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30520 | 3' | -55.3 | NC_006548.1 | + | 12335 | 0.69 | 0.424861 |
Target: 5'- -aGGCCCUGUgcGCCG-ACGGGCg-- -3' miRNA: 3'- cgCCGGGACGuaCGGUaUGUCUGacu -5' |
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30520 | 3' | -55.3 | NC_006548.1 | + | 32464 | 0.69 | 0.424861 |
Target: 5'- gGCGGCgaCgGC-UGCCGgcCAGGCUGAu -3' miRNA: 3'- -CGCCGg-GaCGuACGGUauGUCUGACU- -5' |
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30520 | 3' | -55.3 | NC_006548.1 | + | 15420 | 0.69 | 0.415062 |
Target: 5'- -gGGCauCCUGCAgggUGaCCGUguGCAGGCUGAu -3' miRNA: 3'- cgCCG--GGACGU---AC-GGUA--UGUCUGACU- -5' |
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30520 | 3' | -55.3 | NC_006548.1 | + | 8314 | 0.7 | 0.368221 |
Target: 5'- uGCGGUUCUGCAUGCg--GCuGACgUGAu -3' miRNA: 3'- -CGCCGGGACGUACGguaUGuCUG-ACU- -5' |
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30520 | 3' | -55.3 | NC_006548.1 | + | 17044 | 0.7 | 0.356651 |
Target: 5'- cCGGCuuccagcagcuccgCCUGCAUGCCG-GCGGGCaUGAu -3' miRNA: 3'- cGCCG--------------GGACGUACGGUaUGUCUG-ACU- -5' |
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30520 | 3' | -55.3 | NC_006548.1 | + | 21942 | 0.71 | 0.308986 |
Target: 5'- uGuuGCCCgcgGCGUaGCUAUugGGGCUGAa -3' miRNA: 3'- -CgcCGGGa--CGUA-CGGUAugUCUGACU- -5' |
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30520 | 3' | -55.3 | NC_006548.1 | + | 28038 | 0.74 | 0.189026 |
Target: 5'- cGCGGCCCUGCAggagGCCuAUgguccagccucacaGCAGGCg-- -3' miRNA: 3'- -CGCCGGGACGUa---CGG-UA--------------UGUCUGacu -5' |
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30520 | 3' | -55.3 | NC_006548.1 | + | 16930 | 1.11 | 0.000415 |
Target: 5'- gGCGGCCCUGCAUGCCAUACAGACUGAu -3' miRNA: 3'- -CGCCGGGACGUACGGUAUGUCUGACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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