Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 30520 | 5' | -59.6 | NC_006548.1 | + | 22733 | 0.68 | 0.290334 |
|
Target: 5'- -cUCGUGCCgGCCGGCgAUGaaguGGCc- -3' miRNA: 3'- guAGUACGGgCGGCCG-UACgu--CCGcc -5' |
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| 30520 | 5' | -59.6 | NC_006548.1 | + | 18517 | 0.71 | 0.186236 |
|
Target: 5'- cCGUCGUGgCCGCCGGgucCAUGUcGGCa- -3' miRNA: 3'- -GUAGUACgGGCGGCC---GUACGuCCGcc -5' |
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| 30520 | 5' | -59.6 | NC_006548.1 | + | 27020 | 0.71 | 0.162567 |
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Target: 5'- gAUCAgcagacgGCCCGCaucagGGcCAUGguGGCGGc -3' miRNA: 3'- gUAGUa------CGGGCGg----CC-GUACguCCGCC- -5' |
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| 30520 | 5' | -59.6 | NC_006548.1 | + | 23798 | 0.77 | 0.069671 |
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Target: 5'- --cCAaGCCCGCCGGcCAUGCcGGUGGc -3' miRNA: 3'- guaGUaCGGGCGGCC-GUACGuCCGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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