Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 30521 | 3' | -51.4 | NC_006548.1 | + | 28179 | 0.72 | 0.463488 |
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Target: 5'- -gGCCG--UGCGAGACCU-GCGUGAUg -3' miRNA: 3'- ggCGGCugAUGUUCUGGAaCGUACUG- -5' |
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| 30521 | 3' | -51.4 | NC_006548.1 | + | 29172 | 0.67 | 0.766465 |
|
Target: 5'- aCCGCCGAC--CAGGuagagcaacuuguGCCUcGCAUGGa -3' miRNA: 3'- -GGCGGCUGauGUUC-------------UGGAaCGUACUg -5' |
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| 30521 | 3' | -51.4 | NC_006548.1 | + | 32853 | 0.66 | 0.818812 |
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Target: 5'- cCCGCUGGCgggauuuucgUGCuggGGGCCUUGCGcuGCu -3' miRNA: 3'- -GGCGGCUG----------AUGu--UCUGGAACGUacUG- -5' |
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| 30521 | 3' | -51.4 | NC_006548.1 | + | 35875 | 0.66 | 0.798869 |
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Target: 5'- aUGCCGGCgcgauccGCGcGGCCggcgGCGUGAUa -3' miRNA: 3'- gGCGGCUGa------UGUuCUGGaa--CGUACUG- -5' |
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| 30521 | 3' | -51.4 | NC_006548.1 | + | 36903 | 0.66 | 0.798869 |
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Target: 5'- aCCGCaacCGGCUGCugcAGGCCgucaacUGguUGACu -3' miRNA: 3'- -GGCG---GCUGAUGu--UCUGGa-----ACguACUG- -5' |
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| 30521 | 3' | -51.4 | NC_006548.1 | + | 37349 | 0.7 | 0.620269 |
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Target: 5'- gCUGCCGACUaGCGgccAGGCCgaGgGUGAUa -3' miRNA: 3'- -GGCGGCUGA-UGU---UCUGGaaCgUACUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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