Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30521 | 3' | -51.4 | NC_006548.1 | + | 37349 | 0.7 | 0.620269 |
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Target: 5'- gCUGCCGACUaGCGgccAGGCCgaGgGUGAUa -3' miRNA: 3'- -GGCGGCUGA-UGU---UCUGGaaCgUACUG- -5' |
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| 30521 | 3' | -51.4 | NC_006548.1 | + | 26525 | 0.7 | 0.597115 |
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Target: 5'- cCCGCCGuACUGgGAGACggaggGCuAUGGCg -3' miRNA: 3'- -GGCGGC-UGAUgUUCUGgaa--CG-UACUG- -5' |
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| 30521 | 3' | -51.4 | NC_006548.1 | + | 27413 | 0.7 | 0.585582 |
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Target: 5'- gCGCUGAacaggauCAAGGCCcaGCGUGACg -3' miRNA: 3'- gGCGGCUgau----GUUCUGGaaCGUACUG- -5' |
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| 30521 | 3' | -51.4 | NC_006548.1 | + | 23996 | 0.71 | 0.517618 |
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Target: 5'- uCCaGCCGAU--CAGGGCCUUGCGU-ACg -3' miRNA: 3'- -GG-CGGCUGauGUUCUGGAACGUAcUG- -5' |
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| 30521 | 3' | -51.4 | NC_006548.1 | + | 28179 | 0.72 | 0.463488 |
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Target: 5'- -gGCCG--UGCGAGACCU-GCGUGAUg -3' miRNA: 3'- ggCGGCugAUGUUCUGGAaCGUACUG- -5' |
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| 30521 | 3' | -51.4 | NC_006548.1 | + | 17017 | 1.14 | 0.000777 |
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Target: 5'- aCCGCCGACUACAAGACCUUGCAUGACa -3' miRNA: 3'- -GGCGGCUGAUGUUCUGGAACGUACUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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