Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 30521 | 5' | -58.7 | NC_006548.1 | + | 25061 | 0.7 | 0.255597 |
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Target: 5'- gCGACCGaaGCCAccuAGGUUGGcgUCGGCg -3' miRNA: 3'- -GCUGGUagCGGU---UCCAGCCcgAGCCG- -5' |
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| 30521 | 5' | -58.7 | NC_006548.1 | + | 3124 | 0.7 | 0.254944 |
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Target: 5'- aGAUCGUUGCCAAGGggaaGGCUCugaacaaGGCg -3' miRNA: 3'- gCUGGUAGCGGUUCCagc-CCGAG-------CCG- -5' |
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| 30521 | 5' | -58.7 | NC_006548.1 | + | 25134 | 0.71 | 0.218792 |
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Target: 5'- gGAUCgGUgGCCGGGG-CGGGCUgGGUc -3' miRNA: 3'- gCUGG-UAgCGGUUCCaGCCCGAgCCG- -5' |
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| 30521 | 5' | -58.7 | NC_006548.1 | + | 17503 | 0.73 | 0.167539 |
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Target: 5'- -cGCCAUCGCCGcugcuGGGUCGacGGCugaucgcccUCGGCg -3' miRNA: 3'- gcUGGUAGCGGU-----UCCAGC--CCG---------AGCCG- -5' |
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| 30521 | 5' | -58.7 | NC_006548.1 | + | 6519 | 0.75 | 0.11975 |
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Target: 5'- uCGugCAcgCGCCAGGGcggcgcggguuaCGGGCUCGGUc -3' miRNA: 3'- -GCugGUa-GCGGUUCCa-----------GCCCGAGCCG- -5' |
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| 30521 | 5' | -58.7 | NC_006548.1 | + | 27534 | 0.75 | 0.107665 |
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Target: 5'- -uGCCAUugagCGCCAGGGUCGaGGcCUUGGCc -3' miRNA: 3'- gcUGGUA----GCGGUUCCAGC-CC-GAGCCG- -5' |
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| 30521 | 5' | -58.7 | NC_006548.1 | + | 8208 | 0.66 | 0.472603 |
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Target: 5'- gGAgCAcagUGCCGAGGUCauGGCgcaguUCGGCg -3' miRNA: 3'- gCUgGUa--GCGGUUCCAGc-CCG-----AGCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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