Results 1 - 20 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30523 | 5' | -56.9 | NC_006548.1 | + | 12824 | 0.66 | 0.528606 |
Target: 5'- -cGCGCGAGCGgC-GCgguaagucCGGUgUGGCu -3' miRNA: 3'- gaCGCGCUCGCgGaCGa-------GCUAgAUCG- -5' |
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30523 | 5' | -56.9 | NC_006548.1 | + | 36814 | 0.66 | 0.517824 |
Target: 5'- -gGCGCGGccuuauGCGCCUGgC-CGGgugCUGGUg -3' miRNA: 3'- gaCGCGCU------CGCGGAC-GaGCUa--GAUCG- -5' |
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30523 | 5' | -56.9 | NC_006548.1 | + | 33368 | 0.66 | 0.475643 |
Target: 5'- gCUGCGC-AGCGCCgcaUGCUCcucgcggccggaGGUCU-GCc -3' miRNA: 3'- -GACGCGcUCGCGG---ACGAG------------CUAGAuCG- -5' |
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30523 | 5' | -56.9 | NC_006548.1 | + | 22876 | 0.67 | 0.465364 |
Target: 5'- -aGCGCuGGCugcaGCUUGCUaCGAaCUGGCg -3' miRNA: 3'- gaCGCGcUCG----CGGACGA-GCUaGAUCG- -5' |
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30523 | 5' | -56.9 | NC_006548.1 | + | 24325 | 0.67 | 0.455201 |
Target: 5'- -gGCcgGCGAGCGCCUGCcCaa--UGGCa -3' miRNA: 3'- gaCG--CGCUCGCGGACGaGcuagAUCG- -5' |
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30523 | 5' | -56.9 | NC_006548.1 | + | 26351 | 0.67 | 0.44516 |
Target: 5'- -aGCGCGcGGCGCgCUGgaugUCGGUC-AGCg -3' miRNA: 3'- gaCGCGC-UCGCG-GACg---AGCUAGaUCG- -5' |
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30523 | 5' | -56.9 | NC_006548.1 | + | 31873 | 0.68 | 0.415801 |
Target: 5'- -gGCGa-AGCGCCUGauuUCGGUCUGuGCc -3' miRNA: 3'- gaCGCgcUCGCGGACg--AGCUAGAU-CG- -5' |
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30523 | 5' | -56.9 | NC_006548.1 | + | 27081 | 0.68 | 0.415801 |
Target: 5'- cCUGaUGCGGGcCGUCUGCU-GAUC-GGCg -3' miRNA: 3'- -GAC-GCGCUC-GCGGACGAgCUAGaUCG- -5' |
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30523 | 5' | -56.9 | NC_006548.1 | + | 1133 | 0.68 | 0.406281 |
Target: 5'- uCUGCGCGgccAGCaacGCCUGUgcggCGAUCauccGGCu -3' miRNA: 3'- -GACGCGC---UCG---CGGACGa---GCUAGa---UCG- -5' |
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30523 | 5' | -56.9 | NC_006548.1 | + | 17748 | 0.68 | 0.406281 |
Target: 5'- -gGCGCGGGUGgccaucagaUCUGCUCccguccAUCUAGCu -3' miRNA: 3'- gaCGCGCUCGC---------GGACGAGc-----UAGAUCG- -5' |
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30523 | 5' | -56.9 | NC_006548.1 | + | 9237 | 0.68 | 0.387659 |
Target: 5'- uUGCGCGu-CGCCUGUcgaUUGAUCacgGGCu -3' miRNA: 3'- gACGCGCucGCGGACG---AGCUAGa--UCG- -5' |
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30523 | 5' | -56.9 | NC_006548.1 | + | 25524 | 0.68 | 0.378561 |
Target: 5'- -aGCGCGGaggcGUGCCUcgGCgCGAUCUguGGCg -3' miRNA: 3'- gaCGCGCU----CGCGGA--CGaGCUAGA--UCG- -5' |
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30523 | 5' | -56.9 | NC_006548.1 | + | 34869 | 0.68 | 0.373171 |
Target: 5'- -gGUGCGcGCGCCUgguucaaugcccaugGCUUGGaCUGGCa -3' miRNA: 3'- gaCGCGCuCGCGGA---------------CGAGCUaGAUCG- -5' |
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30523 | 5' | -56.9 | NC_006548.1 | + | 4676 | 0.68 | 0.369607 |
Target: 5'- uUGCGCGgggaaagcuuGGUGCCcaGUUCGggCUGGCc -3' miRNA: 3'- gACGCGC----------UCGCGGa-CGAGCuaGAUCG- -5' |
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30523 | 5' | -56.9 | NC_006548.1 | + | 3686 | 0.69 | 0.360799 |
Target: 5'- aUGaCGCuGAGCGCCgagGCggUGAaCUGGCg -3' miRNA: 3'- gAC-GCG-CUCGCGGa--CGa-GCUaGAUCG- -5' |
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30523 | 5' | -56.9 | NC_006548.1 | + | 25826 | 0.69 | 0.352138 |
Target: 5'- -aGCGgGAGgGCCUugGCUgGG-CUGGCa -3' miRNA: 3'- gaCGCgCUCgCGGA--CGAgCUaGAUCG- -5' |
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30523 | 5' | -56.9 | NC_006548.1 | + | 22764 | 0.69 | 0.352138 |
Target: 5'- uCUGCGCcugaauggcAGCGacgagCUGCUCGAUCgccAGCg -3' miRNA: 3'- -GACGCGc--------UCGCg----GACGAGCUAGa--UCG- -5' |
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30523 | 5' | -56.9 | NC_006548.1 | + | 13743 | 0.69 | 0.343626 |
Target: 5'- -cGCGCG---GCCUGCUCGAU--GGCg -3' miRNA: 3'- gaCGCGCucgCGGACGAGCUAgaUCG- -5' |
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30523 | 5' | -56.9 | NC_006548.1 | + | 24026 | 0.69 | 0.32705 |
Target: 5'- -aGuCGCGAGCGCCUGCcaggccgggCGGUgUccAGCc -3' miRNA: 3'- gaC-GCGCUCGCGGACGa--------GCUAgA--UCG- -5' |
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30523 | 5' | -56.9 | NC_006548.1 | + | 16020 | 0.69 | 0.319786 |
Target: 5'- aCUGCG-GAGCGCaCUGCUgacggccaugccgaCGAguucugggcgcugggUCUGGCg -3' miRNA: 3'- -GACGCgCUCGCG-GACGA--------------GCU---------------AGAUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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