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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 30524 | 5' | -56.6 | NC_006548.1 | + | 17915 | 1.06 | 0.000734 |
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Target: 5'- gGACUACGACGCGGUCCUGGACCACUAc -3' miRNA: 3'- -CUGAUGCUGCGCCAGGACCUGGUGAU- -5' |
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| 30524 | 5' | -56.6 | NC_006548.1 | + | 15531 | 0.71 | 0.262623 |
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Target: 5'- cGACgACGACGUGGccuUCCUGGAgUACg- -3' miRNA: 3'- -CUGaUGCUGCGCC---AGGACCUgGUGau -5' |
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| 30524 | 5' | -56.6 | NC_006548.1 | + | 30382 | 0.71 | 0.276665 |
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Target: 5'- aGCUACGGCGauaccGcCCUGGACUACUAc -3' miRNA: 3'- cUGAUGCUGCgc---CaGGACCUGGUGAU- -5' |
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| 30524 | 5' | -56.6 | NC_006548.1 | + | 11908 | 0.68 | 0.429817 |
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Target: 5'- uGGCUGCGcCGCucgcugaucGUCCUGGGCUGCa- -3' miRNA: 3'- -CUGAUGCuGCGc--------CAGGACCUGGUGau -5' |
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| 30524 | 5' | -56.6 | NC_006548.1 | + | 27501 | 0.66 | 0.501417 |
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Target: 5'- gGGCUGCaauucGACG-GGUCCagGGGCUGCUGu -3' miRNA: 3'- -CUGAUG-----CUGCgCCAGGa-CCUGGUGAU- -5' |
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| 30524 | 5' | -56.6 | NC_006548.1 | + | 18444 | 0.72 | 0.216255 |
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Target: 5'- uGGCaGCGGCGUGGUcuucugcagggcgcCCUGGACCAaCUGu -3' miRNA: 3'- -CUGaUGCUGCGCCA--------------GGACCUGGU-GAU- -5' |
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| 30524 | 5' | -56.6 | NC_006548.1 | + | 28574 | 0.67 | 0.449678 |
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Target: 5'- gGACUGCGucACGCuGGUCCU--GCCAUUGc -3' miRNA: 3'- -CUGAUGC--UGCG-CCAGGAccUGGUGAU- -5' |
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| 30524 | 5' | -56.6 | NC_006548.1 | + | 5745 | 0.66 | 0.501417 |
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Target: 5'- -uCUACGcGCGCaguuGGgugaagCCUGGACCACg- -3' miRNA: 3'- cuGAUGC-UGCG----CCa-----GGACCUGGUGau -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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