Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30525 | 3' | -50.7 | NC_006548.1 | + | 1119 | 0.66 | 0.869205 |
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Target: 5'- aGCGAGCcuGGCCG-UCUGcGcgGccaGCAACg -3' miRNA: 3'- -CGCUCG--CUGGCaAGAC-CuaCa--UGUUG- -5' |
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| 30525 | 3' | -50.7 | NC_006548.1 | + | 32162 | 0.66 | 0.869205 |
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Target: 5'- -aGAGCGGagaUGUUCgGGGUGaugccgGCAACg -3' miRNA: 3'- cgCUCGCUg--GCAAGaCCUACa-----UGUUG- -5' |
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| 30525 | 3' | -50.7 | NC_006548.1 | + | 28791 | 0.67 | 0.833465 |
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Target: 5'- cUGGGCGACC---CUGGAUGcgGCAGa -3' miRNA: 3'- cGCUCGCUGGcaaGACCUACa-UGUUg -5' |
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| 30525 | 3' | -50.7 | NC_006548.1 | + | 26351 | 0.67 | 0.814137 |
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Target: 5'- aGCGcGCGG-CGcgCUGGAUGUcgguCAGCg -3' miRNA: 3'- -CGCuCGCUgGCaaGACCUACAu---GUUG- -5' |
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| 30525 | 3' | -50.7 | NC_006548.1 | + | 36806 | 0.68 | 0.740436 |
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Target: 5'- uGCGAGCGucGCCGagCUGG---UGCGGCu -3' miRNA: 3'- -CGCUCGC--UGGCaaGACCuacAUGUUG- -5' |
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| 30525 | 3' | -50.7 | NC_006548.1 | + | 22661 | 0.75 | 0.378292 |
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Target: 5'- gGCGAGCGgaacgauagcGCCGUcCUGGA-GUugAACg -3' miRNA: 3'- -CGCUCGC----------UGGCAaGACCUaCAugUUG- -5' |
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| 30525 | 3' | -50.7 | NC_006548.1 | + | 23089 | 0.76 | 0.316788 |
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Target: 5'- cGgGAGCGGCCGUagUGGccGUGUACAucACg -3' miRNA: 3'- -CgCUCGCUGGCAagACC--UACAUGU--UG- -5' |
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| 30525 | 3' | -50.7 | NC_006548.1 | + | 18050 | 1.11 | 0.001372 |
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Target: 5'- gGCGAGCGACCGUUCUGGAUGUACAACc -3' miRNA: 3'- -CGCUCGCUGGCAAGACCUACAUGUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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