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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30527 | 5' | -50.1 | NC_006548.1 | + | 7188 | 0.66 | 0.880596 |
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Target: 5'- gGCGcAAGGACuCGGGGCA-GAgccgCCUgGg -3' miRNA: 3'- -CGCuUUCUUG-GCCCUGUuCUa---GGAgU- -5' |
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| 30527 | 5' | -50.1 | NC_006548.1 | + | 3523 | 0.66 | 0.880596 |
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Target: 5'- uCGAAAGAACCuGGGA--AGGccacggcgcugcUCCUCGc -3' miRNA: 3'- cGCUUUCUUGG-CCCUguUCU------------AGGAGU- -5' |
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| 30527 | 5' | -50.1 | NC_006548.1 | + | 9655 | 0.67 | 0.855206 |
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Target: 5'- gGUGGcuGGGugCGGGGCAuggcGGuguUCCUCAc -3' miRNA: 3'- -CGCUu-UCUugGCCCUGU----UCu--AGGAGU- -5' |
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| 30527 | 5' | -50.1 | NC_006548.1 | + | 34708 | 0.67 | 0.836956 |
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Target: 5'- gGCGAuguguGGAUCGaGGACuGGAucgUCCUCGg -3' miRNA: 3'- -CGCUuu---CUUGGC-CCUGuUCU---AGGAGU- -5' |
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| 30527 | 5' | -50.1 | NC_006548.1 | + | 15045 | 0.67 | 0.827462 |
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Target: 5'- cGCcAAGGAuCUGGG-CGAGAUCgUCAu -3' miRNA: 3'- -CGcUUUCUuGGCCCuGUUCUAGgAGU- -5' |
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| 30527 | 5' | -50.1 | NC_006548.1 | + | 27745 | 0.68 | 0.797646 |
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Target: 5'- cGCGAAAGAaaccccggaGCUGGGcaguucccugcGCAcGAUCUUCGa -3' miRNA: 3'- -CGCUUUCU---------UGGCCC-----------UGUuCUAGGAGU- -5' |
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| 30527 | 5' | -50.1 | NC_006548.1 | + | 18273 | 0.73 | 0.492819 |
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Target: 5'- uGCGAcGGcugaccuACCGGGGCGAGGUUgUCGa -3' miRNA: 3'- -CGCUuUCu------UGGCCCUGUUCUAGgAGU- -5' |
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| 30527 | 5' | -50.1 | NC_006548.1 | + | 19277 | 1.11 | 0.001592 |
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Target: 5'- aGCGAAAGAACCGGGACAAGAUCCUCAc -3' miRNA: 3'- -CGCUUUCUUGGCCCUGUUCUAGGAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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