Results 1 - 20 of 68 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30528 | 3' | -56.4 | NC_006548.1 | + | 22153 | 0.66 | 0.567217 |
Target: 5'- uGCaGGAAGGCCaUCgGCAC-CAUGCCc -3' miRNA: 3'- gCGgCUUUUCGG-GGaCGUGaGUGCGG- -5' |
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30528 | 3' | -56.4 | NC_006548.1 | + | 23096 | 0.66 | 0.567217 |
Target: 5'- gGCCGuaguGgCCgUGUACaUCACGCUg -3' miRNA: 3'- gCGGCuuuuCgGGgACGUG-AGUGCGG- -5' |
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30528 | 3' | -56.4 | NC_006548.1 | + | 6535 | 0.66 | 0.567217 |
Target: 5'- gGCCGAAccaGGGUCCaucaucaggaUGCGuggCACGCCg -3' miRNA: 3'- gCGGCUU---UUCGGGg---------ACGUga-GUGCGG- -5' |
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30528 | 3' | -56.4 | NC_006548.1 | + | 4160 | 0.66 | 0.556202 |
Target: 5'- cCGCgGGAGGGUcaCCUUGCAuCUCGucaGCCu -3' miRNA: 3'- -GCGgCUUUUCG--GGGACGU-GAGUg--CGG- -5' |
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30528 | 3' | -56.4 | NC_006548.1 | + | 28143 | 0.66 | 0.55291 |
Target: 5'- gGCCGguGAGUCgggguucaucgacaCCUuCACUCAgGCCg -3' miRNA: 3'- gCGGCuuUUCGG--------------GGAcGUGAGUgCGG- -5' |
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30528 | 3' | -56.4 | NC_006548.1 | + | 24325 | 0.66 | 0.54525 |
Target: 5'- gGCCGgcGAGCgCCUGCccaauggcaaACUgagCAgGCCg -3' miRNA: 3'- gCGGCuuUUCGgGGACG----------UGA---GUgCGG- -5' |
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30528 | 3' | -56.4 | NC_006548.1 | + | 23479 | 0.66 | 0.54525 |
Target: 5'- aCGCCcuGAAaUUCCUGCAgCUCAuCGCCg -3' miRNA: 3'- -GCGGcuUUUcGGGGACGU-GAGU-GCGG- -5' |
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30528 | 3' | -56.4 | NC_006548.1 | + | 18735 | 0.66 | 0.54525 |
Target: 5'- aGCCGc--AGgCCgUGCuGCUCgACGCCa -3' miRNA: 3'- gCGGCuuuUCgGGgACG-UGAG-UGCGG- -5' |
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30528 | 3' | -56.4 | NC_006548.1 | + | 9088 | 0.66 | 0.54525 |
Target: 5'- gCGCCGGAAcuggaagaccugGGUgCC-GCGCUCGC-CCu -3' miRNA: 3'- -GCGGCUUU------------UCGgGGaCGUGAGUGcGG- -5' |
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30528 | 3' | -56.4 | NC_006548.1 | + | 37280 | 0.66 | 0.544159 |
Target: 5'- gGCCGGuguuAGGCCCCauauagagagucaUGCGgaaACGCCu -3' miRNA: 3'- gCGGCUu---UUCGGGG-------------ACGUgagUGCGG- -5' |
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30528 | 3' | -56.4 | NC_006548.1 | + | 14852 | 0.66 | 0.534369 |
Target: 5'- -aCCGAuccGAGCCgCCUGaaGCUCAUGCa -3' miRNA: 3'- gcGGCUu--UUCGG-GGACg-UGAGUGCGg -5' |
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30528 | 3' | -56.4 | NC_006548.1 | + | 35942 | 0.66 | 0.534369 |
Target: 5'- aCGCCGcc-GGCCgC-GCGgaUCGCGCCg -3' miRNA: 3'- -GCGGCuuuUCGGgGaCGUg-AGUGCGG- -5' |
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30528 | 3' | -56.4 | NC_006548.1 | + | 19640 | 0.66 | 0.523565 |
Target: 5'- gCGCCuGAAAGGCCCUacaCGCgugaACGCUg -3' miRNA: 3'- -GCGG-CUUUUCGGGGac-GUGag--UGCGG- -5' |
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30528 | 3' | -56.4 | NC_006548.1 | + | 9937 | 0.66 | 0.523565 |
Target: 5'- cCGCCau--GGCCgCUGCAaccaaagccCUCACgGCCg -3' miRNA: 3'- -GCGGcuuuUCGGgGACGU---------GAGUG-CGG- -5' |
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30528 | 3' | -56.4 | NC_006548.1 | + | 23538 | 0.66 | 0.52034 |
Target: 5'- cCGCUGAccccgccaggcgguGGGGUUCCgcagGUACUgGCGCCc -3' miRNA: 3'- -GCGGCU--------------UUUCGGGGa---CGUGAgUGCGG- -5' |
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30528 | 3' | -56.4 | NC_006548.1 | + | 25772 | 0.67 | 0.512846 |
Target: 5'- aGCC--AAGGCCCUccCGCUCACGUg -3' miRNA: 3'- gCGGcuUUUCGGGGacGUGAGUGCGg -5' |
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30528 | 3' | -56.4 | NC_006548.1 | + | 20449 | 0.67 | 0.512846 |
Target: 5'- aGCUGGucGGCCCguucgGCGCcuUCGCGCUc -3' miRNA: 3'- gCGGCUuuUCGGGga---CGUG--AGUGCGG- -5' |
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30528 | 3' | -56.4 | NC_006548.1 | + | 4339 | 0.67 | 0.512846 |
Target: 5'- aCGCCGGAGcuGGCCCgaUGCcUggAUGCCg -3' miRNA: 3'- -GCGGCUUU--UCGGGg-ACGuGagUGCGG- -5' |
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30528 | 3' | -56.4 | NC_006548.1 | + | 7132 | 0.67 | 0.502218 |
Target: 5'- uGCCcc-GAGUCCUUGCgccGCUUACGCg -3' miRNA: 3'- gCGGcuuUUCGGGGACG---UGAGUGCGg -5' |
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30528 | 3' | -56.4 | NC_006548.1 | + | 36102 | 0.67 | 0.502218 |
Target: 5'- gCGCCGGAAagaucgacuauGGCaCCCUcacgagggGCAC-CAuCGCCa -3' miRNA: 3'- -GCGGCUUU-----------UCG-GGGA--------CGUGaGU-GCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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