Results 1 - 20 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30530 | 3' | -55.3 | NC_006548.1 | + | 8206 | 0.66 | 0.66409 |
Target: 5'- aGCAcagugcCGAGGUCAUggCGcAGUUCGgCGAc -3' miRNA: 3'- gCGUu-----GCUCCAGUG--GC-UCAAGCgGCU- -5' |
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30530 | 3' | -55.3 | NC_006548.1 | + | 7832 | 0.66 | 0.66409 |
Target: 5'- uGCAACGAGaucGUCAUCaucUUCGCUGAu -3' miRNA: 3'- gCGUUGCUC---CAGUGGcucAAGCGGCU- -5' |
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30530 | 3' | -55.3 | NC_006548.1 | + | 26038 | 0.66 | 0.652943 |
Target: 5'- gGCGgacAUGAGGUCuCCGGGcaagCGCuCGAu -3' miRNA: 3'- gCGU---UGCUCCAGuGGCUCaa--GCG-GCU- -5' |
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30530 | 3' | -55.3 | NC_006548.1 | + | 35883 | 0.66 | 0.6306 |
Target: 5'- uGUGAUGGGGUCAUgccagacgacaaCGAacugGUUCGCCGu -3' miRNA: 3'- gCGUUGCUCCAGUG------------GCU----CAAGCGGCu -5' |
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30530 | 3' | -55.3 | NC_006548.1 | + | 21026 | 0.66 | 0.619426 |
Target: 5'- aGCuc--AGGUCGCCGccgUCGCCGGa -3' miRNA: 3'- gCGuugcUCCAGUGGCucaAGCGGCU- -5' |
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30530 | 3' | -55.3 | NC_006548.1 | + | 26999 | 0.66 | 0.608263 |
Target: 5'- aGCAGCG---UCGgCGAGUcCGCCGAu -3' miRNA: 3'- gCGUUGCuccAGUgGCUCAaGCGGCU- -5' |
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30530 | 3' | -55.3 | NC_006548.1 | + | 20281 | 0.67 | 0.58601 |
Target: 5'- gGCGGCacGG-CACCGAGgaaGCCGAu -3' miRNA: 3'- gCGUUGcuCCaGUGGCUCaagCGGCU- -5' |
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30530 | 3' | -55.3 | NC_006548.1 | + | 23594 | 0.67 | 0.574938 |
Target: 5'- cCGCcuGGCGGGGUCAgCGGGUcaUCcggcCCGAg -3' miRNA: 3'- -GCG--UUGCUCCAGUgGCUCA--AGc---GGCU- -5' |
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30530 | 3' | -55.3 | NC_006548.1 | + | 14468 | 0.67 | 0.563915 |
Target: 5'- aCGCAGCGAGG-CGCUGA---CGgCGAu -3' miRNA: 3'- -GCGUUGCUCCaGUGGCUcaaGCgGCU- -5' |
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30530 | 3' | -55.3 | NC_006548.1 | + | 23263 | 0.67 | 0.552949 |
Target: 5'- aCGCAGCaAGG-CAUCGAgcaGUUCGCUGu -3' miRNA: 3'- -GCGUUGcUCCaGUGGCU---CAAGCGGCu -5' |
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30530 | 3' | -55.3 | NC_006548.1 | + | 32291 | 0.67 | 0.540962 |
Target: 5'- -uCGACcAGGUCggacuggugaacuGCCGAGUaCGCCGAg -3' miRNA: 3'- gcGUUGcUCCAG-------------UGGCUCAaGCGGCU- -5' |
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30530 | 3' | -55.3 | NC_006548.1 | + | 35309 | 0.68 | 0.531219 |
Target: 5'- cCGCcaguCGAGGauUCACCGguugguccGGggCGCCGAa -3' miRNA: 3'- -GCGuu--GCUCC--AGUGGC--------UCaaGCGGCU- -5' |
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30530 | 3' | -55.3 | NC_006548.1 | + | 19917 | 0.68 | 0.509806 |
Target: 5'- uCGCucuCGAuGGUCGCCcggugGAGUUCaCCGAg -3' miRNA: 3'- -GCGuu-GCU-CCAGUGG-----CUCAAGcGGCU- -5' |
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30530 | 3' | -55.3 | NC_006548.1 | + | 20289 | 0.68 | 0.509806 |
Target: 5'- uGaCGGCGcccuGGcCAUCGAGUUCGCgGAa -3' miRNA: 3'- gC-GUUGCu---CCaGUGGCUCAAGCGgCU- -5' |
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30530 | 3' | -55.3 | NC_006548.1 | + | 22768 | 0.69 | 0.478394 |
Target: 5'- aGCGGCGGaGUCGCCuuugcGGaUCGCCGAc -3' miRNA: 3'- gCGUUGCUcCAGUGGc----UCaAGCGGCU- -5' |
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30530 | 3' | -55.3 | NC_006548.1 | + | 17454 | 0.69 | 0.478394 |
Target: 5'- aGCGGCGAuGG-CGCCGcAGcUgGCCGAa -3' miRNA: 3'- gCGUUGCU-CCaGUGGC-UCaAgCGGCU- -5' |
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30530 | 3' | -55.3 | NC_006548.1 | + | 27859 | 0.69 | 0.457991 |
Target: 5'- uGCAGCGcaguGGUgGCCGAGgcgaCGCCu- -3' miRNA: 3'- gCGUUGCu---CCAgUGGCUCaa--GCGGcu -5' |
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30530 | 3' | -55.3 | NC_006548.1 | + | 13415 | 0.69 | 0.457991 |
Target: 5'- gGCGGCGggccAGGUCGCUGuuUgagCGCCGGc -3' miRNA: 3'- gCGUUGC----UCCAGUGGCucAa--GCGGCU- -5' |
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30530 | 3' | -55.3 | NC_006548.1 | + | 6532 | 0.69 | 0.438062 |
Target: 5'- gGCAuCGAGGUCAUCGuGcacgCGCCa- -3' miRNA: 3'- gCGUuGCUCCAGUGGCuCaa--GCGGcu -5' |
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30530 | 3' | -55.3 | NC_006548.1 | + | 20092 | 0.7 | 0.39051 |
Target: 5'- cCGC-ACcAGGUggUGCCgGAGUUCGCCGAg -3' miRNA: 3'- -GCGuUGcUCCA--GUGG-CUCAAGCGGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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