Results 1 - 20 of 53 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30531 | 3' | -57.2 | NC_006548.1 | + | 6916 | 0.66 | 0.497611 |
Target: 5'- aUGCagGCGgUAgcugUGCAGCGCGcggcugaucGCGCu -3' miRNA: 3'- -ACGagUGCgGUa---ACGUCGCGCu--------CGCG- -5' |
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30531 | 3' | -57.2 | NC_006548.1 | + | 29650 | 0.66 | 0.487066 |
Target: 5'- gUGC-CGCuGCCA--GCGGUGCcGGUGCg -3' miRNA: 3'- -ACGaGUG-CGGUaaCGUCGCGcUCGCG- -5' |
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30531 | 3' | -57.2 | NC_006548.1 | + | 14086 | 0.66 | 0.487066 |
Target: 5'- aGCUCGCGgCGgaucuccaGCGGUGUG-GCGUc -3' miRNA: 3'- aCGAGUGCgGUaa------CGUCGCGCuCGCG- -5' |
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30531 | 3' | -57.2 | NC_006548.1 | + | 25852 | 0.66 | 0.487066 |
Target: 5'- cUGCUaggcuCGCCcgUGCucaCGUGAGCGg -3' miRNA: 3'- -ACGAgu---GCGGuaACGuc-GCGCUCGCg -5' |
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30531 | 3' | -57.2 | NC_006548.1 | + | 22681 | 0.66 | 0.487066 |
Target: 5'- aGUUC-CGCUuccgGCAGUucgGCGAGCGg -3' miRNA: 3'- aCGAGuGCGGuaa-CGUCG---CGCUCGCg -5' |
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30531 | 3' | -57.2 | NC_006548.1 | + | 17518 | 0.66 | 0.47559 |
Target: 5'- cUGCgCACGCuCAUcgagcaggcaaGCAgcuuggaagaucuGCGCGAGCGCc -3' miRNA: 3'- -ACGaGUGCG-GUAa----------CGU-------------CGCGCUCGCG- -5' |
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30531 | 3' | -57.2 | NC_006548.1 | + | 26301 | 0.66 | 0.456092 |
Target: 5'- cGCgccgCGCGCUuuuacuaccUGCAGCa-GAGCGCg -3' miRNA: 3'- aCGa---GUGCGGua-------ACGUCGcgCUCGCG- -5' |
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30531 | 3' | -57.2 | NC_006548.1 | + | 25786 | 0.66 | 0.456092 |
Target: 5'- cGCUCACGUgA--GCAcGgGCGAGCcuaGCa -3' miRNA: 3'- aCGAGUGCGgUaaCGU-CgCGCUCG---CG- -5' |
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30531 | 3' | -57.2 | NC_006548.1 | + | 14625 | 0.66 | 0.456092 |
Target: 5'- cGCUgACGCCA--GCAGUcCGA-CGCg -3' miRNA: 3'- aCGAgUGCGGUaaCGUCGcGCUcGCG- -5' |
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30531 | 3' | -57.2 | NC_006548.1 | + | 22771 | 0.66 | 0.446004 |
Target: 5'- aGCaccuUCuGCGCCugaaugGCAGCGaCGAGCuGCu -3' miRNA: 3'- aCG----AG-UGCGGuaa---CGUCGC-GCUCG-CG- -5' |
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30531 | 3' | -57.2 | NC_006548.1 | + | 34982 | 0.66 | 0.446004 |
Target: 5'- aGC-CACGCacgUGCGGagGCGAGCa- -3' miRNA: 3'- aCGaGUGCGguaACGUCg-CGCUCGcg -5' |
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30531 | 3' | -57.2 | NC_006548.1 | + | 15013 | 0.67 | 0.425234 |
Target: 5'- cUGCUCAC-CCAUUGauccacucGCGCGGacucggcagcaguGCGCu -3' miRNA: 3'- -ACGAGUGcGGUAACgu------CGCGCU-------------CGCG- -5' |
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30531 | 3' | -57.2 | NC_006548.1 | + | 3682 | 0.67 | 0.41651 |
Target: 5'- cGCUgaGCGCCGagGCGGUGaacuGGCGCu -3' miRNA: 3'- aCGAg-UGCGGUaaCGUCGCgc--UCGCG- -5' |
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30531 | 3' | -57.2 | NC_006548.1 | + | 26402 | 0.67 | 0.41651 |
Target: 5'- aGCUCugGCCAUcgacccgGCcGC-CGAacGCGCu -3' miRNA: 3'- aCGAGugCGGUAa------CGuCGcGCU--CGCG- -5' |
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30531 | 3' | -57.2 | NC_006548.1 | + | 16334 | 0.67 | 0.41651 |
Target: 5'- cUGCa-GCGCCGUgGCGGCaacgaccuGCGGGUGUa -3' miRNA: 3'- -ACGagUGCGGUAaCGUCG--------CGCUCGCG- -5' |
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30531 | 3' | -57.2 | NC_006548.1 | + | 13376 | 0.67 | 0.397522 |
Target: 5'- gGCgCGCGCUccUGCGGCGgGcaaaccGGCGCc -3' miRNA: 3'- aCGaGUGCGGuaACGUCGCgC------UCGCG- -5' |
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30531 | 3' | -57.2 | NC_006548.1 | + | 37408 | 0.67 | 0.397522 |
Target: 5'- gGC-CugGCCGcuagucgGCAGCGUGGcgacGCGCc -3' miRNA: 3'- aCGaGugCGGUaa-----CGUCGCGCU----CGCG- -5' |
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30531 | 3' | -57.2 | NC_006548.1 | + | 23057 | 0.67 | 0.397522 |
Target: 5'- cUGCUCAguugcUGCCGUugggUGCaaucgAGCGgGAGCGg -3' miRNA: 3'- -ACGAGU-----GCGGUA----ACG-----UCGCgCUCGCg -5' |
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30531 | 3' | -57.2 | NC_006548.1 | + | 16807 | 0.68 | 0.388239 |
Target: 5'- gGCUCAuCGUCGUUGuCGGCGCcGGuCGa -3' miRNA: 3'- aCGAGU-GCGGUAAC-GUCGCGcUC-GCg -5' |
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30531 | 3' | -57.2 | NC_006548.1 | + | 27270 | 0.68 | 0.3791 |
Target: 5'- uUGCUCGguCGCCGccUGCuGGaCGCGAGCa- -3' miRNA: 3'- -ACGAGU--GCGGUa-ACG-UC-GCGCUCGcg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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