Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30531 | 5' | -57.1 | NC_006548.1 | + | 18444 | 0.66 | 0.473927 |
Target: 5'- uGGCAGCGGCGuGGUcuucugcaGGGCGCCcUGg -3' miRNA: 3'- cUUGUCGCCGU-CCGa-------CCUGCGGuAUa -5' |
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30531 | 5' | -57.1 | NC_006548.1 | + | 23225 | 0.67 | 0.384776 |
Target: 5'- uGGAUuGcCGGUAGaucGCUGGACGCCAg-- -3' miRNA: 3'- -CUUGuC-GCCGUC---CGACCUGCGGUaua -5' |
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30531 | 5' | -57.1 | NC_006548.1 | + | 26352 | 0.67 | 0.384776 |
Target: 5'- aAGCGcGCGGCGcGCUGGAUGUCGg-- -3' miRNA: 3'- cUUGU-CGCCGUcCGACCUGCGGUaua -5' |
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30531 | 5' | -57.1 | NC_006548.1 | + | 18006 | 0.67 | 0.366523 |
Target: 5'- --cCAGCuauGC-GGCUGGACGCUAUGc -3' miRNA: 3'- cuuGUCGc--CGuCCGACCUGCGGUAUa -5' |
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30531 | 5' | -57.1 | NC_006548.1 | + | 13421 | 0.67 | 0.366523 |
Target: 5'- aGAAcCGGCGGCGGGCcaGGuCGCUGUu- -3' miRNA: 3'- -CUU-GUCGCCGUCCGa-CCuGCGGUAua -5' |
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30531 | 5' | -57.1 | NC_006548.1 | + | 30679 | 0.68 | 0.356741 |
Target: 5'- cGACGGCGGaCAGGgUGGcaggaugaagcccGCGCCGg-- -3' miRNA: 3'- cUUGUCGCC-GUCCgACC-------------UGCGGUaua -5' |
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30531 | 5' | -57.1 | NC_006548.1 | + | 16509 | 0.68 | 0.348875 |
Target: 5'- -uGCAGCGcGUGGGCUGGgaccGCGUCAc-- -3' miRNA: 3'- cuUGUCGC-CGUCCGACC----UGCGGUaua -5' |
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30531 | 5' | -57.1 | NC_006548.1 | + | 3582 | 0.68 | 0.348875 |
Target: 5'- cAACuGGUGcCGGGCUGGugGCCGUu- -3' miRNA: 3'- cUUG-UCGCcGUCCGACCugCGGUAua -5' |
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30531 | 5' | -57.1 | NC_006548.1 | + | 32468 | 0.69 | 0.315426 |
Target: 5'- cAACGGCGGCGacGGCUGccggccaggcuGAUGCCAUc- -3' miRNA: 3'- cUUGUCGCCGU--CCGAC-----------CUGCGGUAua -5' |
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30531 | 5' | -57.1 | NC_006548.1 | + | 20582 | 0.69 | 0.291968 |
Target: 5'- cAGCGGCGGCagAGGCUGcugcggcgucGACGCCu--- -3' miRNA: 3'- cUUGUCGCCG--UCCGAC----------CUGCGGuaua -5' |
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30531 | 5' | -57.1 | NC_006548.1 | + | 36433 | 0.69 | 0.284457 |
Target: 5'- uGAGCAGCGcguaguucaucuGCAGGCcGGACgGCCGc-- -3' miRNA: 3'- -CUUGUCGC------------CGUCCGaCCUG-CGGUaua -5' |
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30531 | 5' | -57.1 | NC_006548.1 | + | 13662 | 0.7 | 0.254578 |
Target: 5'- uGAACAGCucuccuaccGGCUGGACGCCAUc- -3' miRNA: 3'- -CUUGUCGccgu-----CCGACCUGCGGUAua -5' |
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30531 | 5' | -57.1 | NC_006548.1 | + | 35198 | 0.7 | 0.242582 |
Target: 5'- cGAgGGCGGCAucguugggccGCUGGACGUCAUGUu -3' miRNA: 3'- cUUgUCGCCGUc---------CGACCUGCGGUAUA- -5' |
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30531 | 5' | -57.1 | NC_006548.1 | + | 21035 | 1.05 | 0.000595 |
Target: 5'- aGAACAGCGGCAGGCUGGACGCCAUAUc -3' miRNA: 3'- -CUUGUCGCCGUCCGACCUGCGGUAUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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