Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30533 | 3' | -58.2 | NC_006548.1 | + | 28691 | 0.66 | 0.406281 |
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Target: 5'- aGGAAGcgGCGACCUaCGgCaAGGGCc- -3' miRNA: 3'- aCCUUCa-UGCUGGA-GCgGgUCCCGaa -5' |
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| 30533 | 3' | -58.2 | NC_006548.1 | + | 9079 | 0.66 | 0.39504 |
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Target: 5'- cUGGAagaccugGGUGCcGCgCUCGCCCugugcauccauugAGGGCUg -3' miRNA: 3'- -ACCU-------UCAUGcUG-GAGCGGG-------------UCCCGAa -5' |
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| 30533 | 3' | -58.2 | NC_006548.1 | + | 8442 | 0.67 | 0.37856 |
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Target: 5'- cGGGugauGUugGccggGCuCUCGCCUAGGGCc- -3' miRNA: 3'- aCCUu---CAugC----UG-GAGCGGGUCCCGaa -5' |
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| 30533 | 3' | -58.2 | NC_006548.1 | + | 28768 | 0.67 | 0.352138 |
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Target: 5'- cUGGAAGccCGugCUCGCgCCGccuGGGCg- -3' miRNA: 3'- -ACCUUCauGCugGAGCG-GGU---CCCGaa -5' |
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| 30533 | 3' | -58.2 | NC_006548.1 | + | 31660 | 0.68 | 0.295696 |
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Target: 5'- cUGGAug-GCGGCCccUCGCCCgAGuGGCUg -3' miRNA: 3'- -ACCUucaUGCUGG--AGCGGG-UC-CCGAa -5' |
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| 30533 | 3' | -58.2 | NC_006548.1 | + | 1102 | 0.69 | 0.259859 |
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Target: 5'- aUGGAGGUguuccAUGGCaCUCGCUagGGGGCUg -3' miRNA: 3'- -ACCUUCA-----UGCUG-GAGCGGg-UCCCGAa -5' |
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| 30533 | 3' | -58.2 | NC_006548.1 | + | 33113 | 0.69 | 0.253131 |
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Target: 5'- cGGAGGUcAUGcCCUCGacaacauaCCGGGGCUg -3' miRNA: 3'- aCCUUCA-UGCuGGAGCg-------GGUCCCGAa -5' |
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| 30533 | 3' | -58.2 | NC_006548.1 | + | 4229 | 0.71 | 0.198875 |
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Target: 5'- uUGGAAugcCGGCCUUGCgCAGGGCg- -3' miRNA: 3'- -ACCUUcauGCUGGAGCGgGUCCCGaa -5' |
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| 30533 | 3' | -58.2 | NC_006548.1 | + | 12640 | 0.71 | 0.188282 |
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Target: 5'- cGGAAGUugGCGGCCgagCGCCCGGcGUUg -3' miRNA: 3'- aCCUUCA--UGCUGGa--GCGGGUCcCGAa -5' |
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| 30533 | 3' | -58.2 | NC_006548.1 | + | 21625 | 1.04 | 0.000575 |
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Target: 5'- cUGGAAGUACGACCUCGCCCAGGGCUUc -3' miRNA: 3'- -ACCUUCAUGCUGGAGCGGGUCCCGAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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