Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30533 | 5' | -56.3 | NC_006548.1 | + | 16861 | 0.66 | 0.582151 |
Target: 5'- cGGuauGCCCacggcGGUCGGCCGCuaugacgacAAGaCCGc -3' miRNA: 3'- -CCuu-UGGGa----CCAGCCGGCG---------UUCaGGU- -5' |
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30533 | 5' | -56.3 | NC_006548.1 | + | 33310 | 0.66 | 0.57108 |
Target: 5'- gGGAAGCCaacccccgcCUGGUCGGCaaauuGCGgcAGcCCGg -3' miRNA: 3'- -CCUUUGG---------GACCAGCCGg----CGU--UCaGGU- -5' |
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30533 | 5' | -56.3 | NC_006548.1 | + | 35586 | 0.66 | 0.56006 |
Target: 5'- --uGACgCCUGGUCcagcaGGCCGCGA-UCCu -3' miRNA: 3'- ccuUUG-GGACCAG-----CCGGCGUUcAGGu -5' |
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30533 | 5' | -56.3 | NC_006548.1 | + | 6588 | 0.66 | 0.538209 |
Target: 5'- -uGGACCCUGGuUCGGCCaacaucucgGCGAuGUCgAa -3' miRNA: 3'- ccUUUGGGACC-AGCCGG---------CGUU-CAGgU- -5' |
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30533 | 5' | -56.3 | NC_006548.1 | + | 17888 | 0.67 | 0.515588 |
Target: 5'- cGGcauGACCCUGG-CGGaguuCCGCAaggacuacgacgcGGUCCu -3' miRNA: 3'- -CCu--UUGGGACCaGCC----GGCGU-------------UCAGGu -5' |
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30533 | 5' | -56.3 | NC_006548.1 | + | 7621 | 0.68 | 0.464434 |
Target: 5'- ---cGCgCUGGUCGGCCGUcauGGuuUCCAc -3' miRNA: 3'- ccuuUGgGACCAGCCGGCGu--UC--AGGU- -5' |
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30533 | 5' | -56.3 | NC_006548.1 | + | 18508 | 0.69 | 0.387083 |
Target: 5'- --uGACCC-GGUCcgucguGGCCGCcGGGUCCAu -3' miRNA: 3'- ccuUUGGGaCCAG------CCGGCG-UUCAGGU- -5' |
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30533 | 5' | -56.3 | NC_006548.1 | + | 13085 | 0.69 | 0.384351 |
Target: 5'- aGGAcGCCCUGGUCcaguacaagcagcaGGUCGCGcGcgCCAa -3' miRNA: 3'- -CCUuUGGGACCAG--------------CCGGCGUuCa-GGU- -5' |
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30533 | 5' | -56.3 | NC_006548.1 | + | 30520 | 0.7 | 0.343245 |
Target: 5'- uGGAAccagccgauGCCCuacUGGUucuucgccaaCGGCCGCAGGUUCu -3' miRNA: 3'- -CCUU---------UGGG---ACCA----------GCCGGCGUUCAGGu -5' |
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30533 | 5' | -56.3 | NC_006548.1 | + | 29241 | 0.7 | 0.342405 |
Target: 5'- cGAGGCacaaguugcucuaCCUGGUCGGCgguagaccgaUGCAGGUCUAg -3' miRNA: 3'- cCUUUG-------------GGACCAGCCG----------GCGUUCAGGU- -5' |
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30533 | 5' | -56.3 | NC_006548.1 | + | 19078 | 0.71 | 0.310829 |
Target: 5'- uGGguGCCCUGG-CGGCCGgGAaaucgCCAg -3' miRNA: 3'- -CCuuUGGGACCaGCCGGCgUUca---GGU- -5' |
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30533 | 5' | -56.3 | NC_006548.1 | + | 21690 | 0.73 | 0.233854 |
Target: 5'- uGAAGCCCUGGgcgaGGUCGUAcuUCCAg -3' miRNA: 3'- cCUUUGGGACCag--CCGGCGUucAGGU- -5' |
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30533 | 5' | -56.3 | NC_006548.1 | + | 9843 | 0.76 | 0.138804 |
Target: 5'- cGGAAGCguugcuucaCCUGGUCGGCCGUGAGggCUu -3' miRNA: 3'- -CCUUUG---------GGACCAGCCGGCGUUCa-GGu -5' |
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30533 | 5' | -56.3 | NC_006548.1 | + | 21660 | 1.11 | 0.000361 |
Target: 5'- cGGAAACCCUGGUCGGCCGCAAGUCCAa -3' miRNA: 3'- -CCUUUGGGACCAGCCGGCGUUCAGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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