Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 30539 | 3' | -54.7 | NC_006548.1 | + | 8207 | 0.69 | 0.441155 |
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Target: 5'- gAGCaCAgUGCCGAGgucauGGCGcAGUUCGGCg -3' miRNA: 3'- -UCGaGUaGCGGCUC-----CCGU-UCAAGUCG- -5' |
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| 30539 | 3' | -54.7 | NC_006548.1 | + | 12408 | 0.7 | 0.373857 |
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Target: 5'- cGGCUgGUuggUGCCGAGGGCGgccggauacAGgcCGGCg -3' miRNA: 3'- -UCGAgUA---GCGGCUCCCGU---------UCaaGUCG- -5' |
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| 30539 | 3' | -54.7 | NC_006548.1 | + | 12335 | 0.71 | 0.355949 |
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Target: 5'- aGGCcCugugCGCCGAcGGGCGAGcgggcaUCAGCa -3' miRNA: 3'- -UCGaGua--GCGGCU-CCCGUUCa-----AGUCG- -5' |
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| 30539 | 3' | -54.7 | NC_006548.1 | + | 14499 | 0.76 | 0.154629 |
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Target: 5'- cAGgUCGUCGCCGccuGGGCAcGGaUCAGCu -3' miRNA: 3'- -UCgAGUAGCGGCu--CCCGU-UCaAGUCG- -5' |
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| 30539 | 3' | -54.7 | NC_006548.1 | + | 1681 | 0.66 | 0.638597 |
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Target: 5'- aAGCUgCAUUggGCCG-GGGCGgagagAGUuaUCGGCu -3' miRNA: 3'- -UCGA-GUAG--CGGCuCCCGU-----UCA--AGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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